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Die Umstände des globalen Klimawandels erfordern zukünftig auch in Hannover eine Neuauswahl der Stadtbaumarten zugunsten hitze- und trockenheitstoleranter Arten. In Zusammenarbeit mit der Stadt Hannover wurde ein Baumentwicklungskonzept für zukünftige Baumpflanzungen in der Landeshauptstadt erarbeitet. Im Konflikt zwischen Schutz der heimischen Flora und Fauna und Maßnahmen der Klimaanpassung wurde die „Assisted Migration“ als Ansatz und Kompromiss zwischen beiden Bereichen gewählt. Hierbei werden Arten durch gezielte Eingriffe parallel zum Verlauf der theoretischer Arealverschiebung der Klimaerwärmung bewegt. Um mögliche Herkunftsgebiete für diese Arten zu finden, wurden auf europäischer Ebene klimatische Analoggebiete für Hannovers zukünftiges Klima gesucht. Das Klima verschiedener Standorttypen Hannovers wurde mithilfe von aktuellen klimatischen Szenarios für den Zeitraum 2080-2100 projektiert und anschließend europaweit mit dem lokalen Klima der Periode 1970-2000 verglichen. Gebiete Süd- und Südosteuropas verfügen über klimatischen Eigenschaften, welche dem in Hannover zukünftig erwarteten Klima entsprechen. Sie wurden auf ihr Gehölzvorkommen untersucht. Unter Einbezug der natürlichen Standortbedingungen und bereits gemachter Erfahrungen wurde eine umfangreiche Artenliste erarbeitet. Diese schlägt Baumarten vor, die zukünftig an die Klimabedingungen angepasst sind und jetzt und im Verlauf der nächsten Jahrzehnte an verschiedenen Standorten Hannovers gepflanzt werden können.
Introduction: Patients undergoing revision total hip surgery (RTHS) have a high prevalence of mild and moderate preoperative anemia, associated with adverse outcomes. The aim of this study was to investigate the association of perioperative allogeneic blood transfusions (ABT) and postoperative complications in preoperatively mild compared to moderate anemic patients undergoing RTHS who did not receive a diagnostic anemia workup and treatment before surgery. Methods: We included 1,765 patients between 2007 and 2019 at a university hospital. Patients were categorized according to their severity of anemia using the WHO criteria of mild, moderate, and severe anemia in the first Hb level of the case. Patients were grouped as having received no ABT, 1–2 units of ABT, or more than 2 units of ABT. Need for intraoperative ABT was assessed in accordance with institutional standards. Primary endpoint was the compound incidence of postoperative complications. Secondary outcomes included major/minor complications and length of hospital and ICU stay. Results: Of the 1,765 patients, 31.0% were anemic of any cause before surgery. Transfusion rates were 81% in anemic patients and 41.2% in nonanemic patients. The adjusted risks for compound postoperative complication were significantly higher in patients with moderate anemia (OR 4.88, 95% CI: 1.54–13.15, p = 0.003) but not for patients with mild anemia (OR 1.93, 95% CI: 0.85–3.94, p < 0.090). Perioperative ABT was associated with significantly higher risks for complications in nonanemic patients and showed an increased risk for complications in all anemic patients. In RTHS, perioperative ABT as a treatment for moderate preoperative anemia of any cause was associated with a negative compound effect on postoperative complications, compared to anemia or ABT alone. Discussion: ABT is associated with adverse outcomes of patients with moderate preoperative anemia before RTHS. For this reason, medical treatment of moderate preoperative anemia may be considered.
Background
Beta-blocker (BB) therapy plays a central role in the treatment of cardiovascular diseases. An increasing number of patients with cardiovascular diseases undergoe noncardiac surgery, where opioids are an integral part of the anesthesiological management. There is evidence to suggest that short-term intravenous BB therapy may influence perioperative opioid requirements due to an assumed cross-talk between G-protein coupled beta-adrenergic and opioid receptors. Whether chronic BB therapy could also have an influence on perioperative opioid requirements is unclear.
Methods
A post hoc analysis of prospectively collected data from a multicenter observational (BioCog) study was performed. Inclusion criteria consisted of elderly patients (≥ 65 years) undergoing elective noncardiac surgery as well as total intravenous general anesthesia without the use of regional anesthesia and duration of anesthesia ≥ 60 min. Two groups were defined: patients with and without BB in their regular preopreative medication. The administered opioids were converted to their respective morphine equivalent doses. Multiple regression analysis was performed using the morphine-index to identify independent predictors.
Results
A total of 747 patients were included in the BioCog study in the study center Berlin. 106 patients fulfilled the inclusion criteria. Of these, 37 were on chronic BB. The latter were preoperatively significantly more likely to have arterial hypertension (94.6%), chronic renal failure (27%) and hyperlipoproteinemia (51.4%) compared to patients without BB. Both groups did not differ in terms of cumulative perioperative morphine equivalent dose (230.9 (BB group) vs. 214.8 mg (Non-BB group)). Predictive factors for increased morphine-index were older age, male sex, longer duration of anesthesia and surgery of the trunk. In a model with logarithmised morphine index, only gender (female) and duration of anesthesia remained predictive factors.
Conclusions
Chronic BB therapy was not associated with a reduced perioperative opioid consumption.
The development of non-precious metal-based electrodes that actively and stably support the oxygen evolution reaction (OER) in water electrolysis systems remains a challenge, especially at low pH levels. The recently published study has conclusively shown that the addition of haematite to H2 SO4 is a highly effective method of significantly reducing oxygen evolution overpotential and extending anode life. The far superior result is achieved by concentrating oxygen evolution centres on the oxide particles rather than on the electrode. However, unsatisfactory Faradaic efficiencies of the OER and hydrogen evolution reaction (HER) parts as well as the required high haematite load impede applicability and upscaling of this process. Here it is shown that the same performance is achieved with three times less metal oxide powder if NiO/H2 SO4 suspensions are used along with stainless steel anodes. The reason for the enormous improvement in OER performance by adding NiO to the electrolyte is the weakening of the intramolecular O─H bond in the water molecules, which is under the direct influence of the nickel oxide suspended in the electrolyte. The manipulation of bonds in water molecules to increase the tendency of the water to split is a ground-breaking development, as shown in this first example.
Test von Schnellverfahren zur Bestimmung der Benetzungseigenschaften von Kultursubstraten (Abstract)
(2024)
Response of petunia to wood fibre amended peat substrate under ebb-and-flow irrigation (Abstract)
(2024)
Besides yield reduction weeds have positive effects, like enhancing biodiversity or reducing soil erosion. The aim of the work is to enhance biodiversity without yield losses. In growing season 2021/22 a field experiment with winter triticale was conducted to experience the impact of mechanical weed control, sowing date and sowing rate. Especially hoeing had a positive impact on vegetative growth and grain yield while weed growth was inhibited but weed biodiversity stayed equal or increased. A lower seed rate or an earlier sowing date made in general no difference at flowering or harvesting stage.
Inhalt dieser Arbeit ist die Entwicklung und Durchführung eines BIM-Workflows für die Erfassung, Aufbereitung und Weiterbearbeitung von Gelände-Bestandsdaten in der Landschaftsarchitektur. Der entwickelte Workflow basiert auf der Analyse vorliegender BIM-Richtlinien und Leitfäden aus dem Bereich der Infrastrukturplanung. Es wird herausgestellt, welche Anforderungen an Bestandsdaten in BIM gestellt werden. Diese können auf Grund überschneidender Inhalte auf die Fachdisziplin der Freiraumplanung übertragen werden. Der Workflow integriert auf der einen Seite 3D-Bestandsdaten der Vermessungsämter, auf der anderen Seite Vermesserdaten aus der Ingenieurvermessung. Die Daten werden für die Weiterverwendung in BIM-Softwares aufbereitet und an diese übergeben. Auf Grundlage eines Projektbeispiels wird in fünf verschiedenen BIM-Programmen die Erstellung eines Digitalen Geländemodells anhand derselben Punktedatei durchgeführt und dokumentiert. Die erstellten Geländemodelle werden in dasselbe Datenformat exportiert und hinsichtlich ihrer Übereinstimmung und Genauigkeit mittels ausgewählter Vergleichsmethoden geprüft. Abschließend werden einige Werkzeuge des Plug-ins ‚Environment‘ für die Modellierung und Weiterbearbeitung von Geländemodellen in Autodesk Revit getestet und bewertet. Die praktische Umsetzung des entwickelten Workflows wird detailliert im Anhang aufgezeigt.
Dairy farming has been the subject of public debate on animal welfare for a number of years now. Animal welfare discussions on dairy farming often include the demand for more nature connectedness in this area. This study focuses on the divergent perspectives of consumers and scientists on the importance of more nature connectedness for animal welfare strategies in German dairy farming. Within Europe, Germany is the main producer of cow’s milk and an important industry in many rural areas in Germany is dairy farming. The insights presented are based on qualitative interviews with dairy farming and livestock researchers from Germany and Austria. A key finding of this study is that we need to look more closely at the actual content of nature claims in animal welfare debates. The scientists interviewed tend to see idealized conditions in animal welfare discussions with images of nature which in fact seldom lead to improved conditions in dairy farming and, even then, only to a limited extent. The scientists interviewed rate calls for more nature connectedness in dairy farming from the nonagricultural public as anti-modern, complexity-reducing, and normative. Nevertheless, some of the scientists interviewed did have valuable insights into the nonagricultural public’s criticism of dairy farming practices. These scientists argued, however, that animal welfare needs to differentiate between nature connectedness and the innate needs of cattle when it comes to animal welfare strategies. An important conclusion of the study is that more discussion formats are needed to promote the exchange of ideas between different social groups attempting to understand animal welfare in dairy farming.
Die vorliegende Abschlussarbeit befasst sich mit der Arbeitsweise des Building Information Modeling (BIM) in der Landschaftsarchitektur und der Untersuchung von Modellierungsop-tionen für Vegetationsobjekte. Wenngleich Vegetation einen wesentlichen Bestandteil der Planung von Außenanlagen darstellt, gibt es für den allgemeinen Umgang mit Bepflanzung in BIM bisher noch keine ausgereiften Konzepte. Insbesondere die Planung von Stauden-flächen wird in digitalen Modellen bisher kaum thematisiert. Es fehlen darüber hinaus allge-meine Workflows, um die Bepflanzungsplanung in den Prozess des Building Information Modeling zu integrieren. Das vorrangige Ziel dieser Arbeit ist daher die Entwicklung von exemplarischen Arbeitsab-läufen für das Aufgabenfeld der Bepflanzungsplanung innerhalb der 3D-Modellierungssoft-ware Autodesk Revit. Um die Potentiale des Programms bestmöglich zu nutzen, werden zudem die visuellen Programmierwerkzeuge des Softwaremoduls Dynamo verwendet. Die in Dynamo entwickelten benutzerdefinierten Tools ergänzen die Standardfunktionen von Revit und können für spezifische Modellierungsaufgaben wiederholt zum Einsatz kommen. Zum einen basiert die Modellierung in Revit auf den BIM-Grundlagen der Fertigstellungsgrade und Anwendungsfälle. Zum anderen soll durch das Modell ein automatisiertes Ableiten von Bepflanzungsplänen ermöglicht werden. Eine Analyse spezialisierter Softwareprodukte für die ‚Grüne Branche‘ gibt Aufschluss über die möglichen Funktionen der zu erstellenden Skripte in Dynamo. Der Workflow in Revit wird an einem Beispielprojekt veranschaulicht. Anhand eines Entwurfes für die Außenanlagen des Wohnkomplexes ‚Charlie Living‘ in Berlin wird somit ein vereinfachtes Modell angefertigt. Das Vegetationsmodell wird sukzessive für die Fertigstellungsgrade 100 bis 300 detailliert und enthält Bepflanzungselemente für Bäume, Hecken, Stauden und Pflanzflächen. Die Vegetationselemente werden mit zusätzlichen alphanumerischen Daten versehen und abschließend in einem Bepflanzungsplan dargestellt. Der Modellierungsprozess wird insgesamt mit zehn verschiedenen benutzerdefinierten Dynamo-Tools unterstützt und automatisiert. Anhand des Modellierungserfolges konnte eine generelle Eignung der BIM-Software Revit für die Anforderungen der Bepflanzungsplanung festgestellt werden. Die dargelegten Workflows in dieser Abschlussarbeit könnten demnach auch für das Modellieren von Vegetationsobjekten in anderen BIM-Projekten mit vergleichbarer Software Anwendung finden.
Primary Liver Cancers : Connecting the Dots of Cellular Studies and Epidemiology with Metabolomics
(2023)
Liver cancers are rising worldwide. Between molecular and epidemiological studies, a research gap has emerged which might be amenable to the technique of metabolomics. This review investigates the current understanding of liver cancer’s trends, etiology and its correlates with existing literature for hepatocellular carcinoma (HCC), cholangiocarcinoma (CCA) and hepatoblastoma (HB). Among additional factors, the literature reports dysfunction in the tricarboxylic acid metabolism, primarily for HB and HCC, and point mutations and signaling for CCA. All cases require further investigation of upstream and downstream events. All liver cancers reported dysfunction in the WNT/β-catenin and P13K/AKT/mTOR pathways as well as changes in FGFR. Metabolites of IHD1, IDH2, miRNA, purine, Q10, lipids, phosphatidylcholine, phosphatidylethanolamine, acylcarnitine, 2-HG and propionyl-CoA emerged as crucial and there was an attempt to elucidate the WNT/β-catenin and P13K/AKT/mTOR pathways metabolomically.
Grasslands are ubiquitous globally, and their conservation and restoration are critical to combat both the biodiversity and climate crises. There is increasing interest in implementing effective multifunctional grassland restoration to restore biodiversity concomitant with above- and belowground carbon sequestration, delivery of carbon credits and/or integration with land dedicated to solar panels. Other common multifunctional restoration considerations include improved forage value, erosion control, water management, pollinator services, and wildlife habitat provisioning. In addition, many grasslands are global biodiversity hotspots. Nonetheless, relative to their impact, and as compared to forests, the importance of preservation, conservation, and restoration of grasslands has been widely overlooked due to their subtle physiognomy and underappreciated contributions to human and planetary well-being. Ultimately, the global success of carbon sequestration will depend on more complete and effective grassland ecosystem restoration. In this review, supported by examples from across the Western world, we call for more strenuous and unified development of best practices for grassland restoration in three areas of concern: initial site conditions and site preparation; implementation of restoration measures and management; and social context and sustainability. For each area, we identify the primary challenges to grassland restoration and highlight case studies with proven results to derive successful and generalizable solutions.
Advances in high-throughput DNA sequencing have propelled research into the human microbiome and its link to metabolic health. We explore microbiome analysis methods, specifically emphasizing metabolomics, how dietary choices impact the production of microbial metabolites, providing an overview of studies examining the connection between enterotypes and diet, and thus, improvement of personalized dietary recommendations. Acetate, propionate, and butyrate constitute more than 95% of the collective pool of short-chain fatty acids. Conflicting data on acetate’s effects may result from its dynamic signaling, which can vary depending on physiological conditions and metabolic phenotypes. Human studies suggest that propionate has overall anti-obesity effects due to its well-documented chemistry, cellular signaling mechanisms, and various clinical benefits. Butyrate, similar to propionate, has the ability to reduce obesity by stimulating the release of appetite-suppressing hormones and promoting the synthesis of leptin. Tryptophan affects systemic hormone secretion, with indole stimulating the release of GLP-1, which impacts insulin secretion, appetite suppression, and gastric emptying. Bile acids, synthesized from cholesterol in the liver and subsequently modified by gut bacteria, play an essential role in the digestion and absorption of dietary fats and fat-soluble vitamins, but they also interact directly with intestinal microbiota and their metabolites. One study using statistical methods identified primarily two groupings of enterotypes Bacteroides and Ruminococcus. The Prevotella-dominated enterotype, P-type, in humans correlates with vegetarians, high-fiber and carbohydrate-rich diets, and traditional diets. Conversely, individuals who consume diets rich in animal fats and proteins, typical in Western-style diets, often exhibit the Bacteroides-dominated, B-type, enterotype. The P-type showcases efficient hydrolytic enzymes for plant fiber degradation but has limited lipid and protein fermentation capacity. Conversely, the B-type features specialized enzymes tailored for the degradation of animal-derived carbohydrates and proteins, showcasing an enhanced saccharolytic and proteolytic potential. Generally, models excel at predictions but often struggle to fully elucidate why certain substances yield varied responses. These studies provide valuable insights into the potential for personalized dietary recommendations based on enterotypes
SimBO is a flexible framework for optimizing discrete event-driven simulations (DES) using sequential optimization algorithms. While specifically designed for Bayesian Optimization (BO) in the context of DES, SimBO can be applied to any black-box problem with other optimization algorithms. The framework consists of four encapsulated components - the black-box problem, the sequential optimization algorithm, a database for experiment configuration and results, and a web-based graphical user interface - that communicate via well-defined interfaces. Each component can be run in different environments, allowing for cooperation between different hardware- and software configurations. In our research context, SimBO’s architecture enabled BO algorithms to be run on a high-performance cluster with GPU support, while the simulation is executed on a local Windows machine using the Simio simulation software. The framework’s flexibility also makes it suitable for evolving from a research-focused tool to a production-ready, cloud-based optimization tool for modern algorithms.
Iron deficiency is still widespread as a major health problem even in countries with adequate food supply. It mainly affects women but also vegans, vegetarians, and athletes and can lead to various clinical pictures. Biofortification of vitamin C-rich vegetables with iron may be one new approach to face this nutritional challenge. However, so far, little is known about the consumer acceptance of iron-biofortified vegetables, particularly in developed countries. To address this issue, a quantitative survey of 1000 consumers in Germany was conducted. The results showed that depending on the type of vegetable, between 54% and 79% of the respondents were interested in iron-biofortified vegetables. Regression analysis showed a relationship between product acceptance, gender, and area of residence. In addition, relationships were found between consumer preferences for enjoyment, sustainability, and naturalness. Compared to functional food and dietary supplements, 77% of respondents would prefer fresh iron-rich vegetables to improve their iron intake. For a market launch, those iron-rich vegetables appear especially promising, which can additionally be advertised with claims for being rich in vitamin C and cultivated in an environmentally friendly way. Consumers were willing to pay EUR 0.10 to EUR 0.20 more for the iron-biofortified vegetables.
Den autochthonen Baumarten geht es unter den klimatischen Veränderungen des Klimawandels so- wohl in der freien Landschaft als auch im urbanen Raum zunehmend schlechter (vgl. fIetz & burger 2021; mlV.nrW 2022). Gleichzeitig können in natürlichen Waldökosystemen Anpassungsdynamiken an diese veränderten klimatischen Bedingungen im Rahmen der Laurophyllisierung beobachtet werden, bei dem sich die mitteleuropäischen sommergrünen Laubwälder in teilweise immergrünen Hartlaubwaldgesell- schaften entwickeln (s. frey et al. 2010: 41). Grund hierfür sind die klimatischen Veränderungen bedingt durch den Klimawandel und Gartentrends (s. berger 2008; deHnen-scHmutz et al. 2006). Der Klimawandel scheint daher günstige Bedingungen für wärmebedürftige und frostempfindliche Immergrüne zu bieten, die an die zu erwartenden Klimaten gut angepasst sind (s. WIttIg 2008: 20). Die Immergrünen, durch die der Prozess der Laurophyllisierung maßgeblich beeinflusst wird, haben ihr natürliches Verbreitungsgebiet überwiegend in der subtropischen vollhumiden Klimazone. Das Klimaxstadium dieser Klimate ist ein im- mergrüner Lorbeerwald, der als Zonobiom V zusammengefasst wird (s. brecKle & rafIqPoor 2019).
Auch in deutschen Städten ist eine solche Anpassung der Stadtbäume an sich ändernde klimatische Bedingungen notwendig. Neben dem Klimawandel spielt im urbanen Raum das Stadtklima eine wich- tige Rolle, welches die Standortbedingungen der Pflanzen zusätzlich beeinflusst und die Auswirkungen des Klimawandels verstärkt (s. HennInger & Weber 2020; WeIscHet & endlIcHer 2018; Kuttler 1998). Die vorlie- gende Arbeit soll daher untersuchen, inwiefern sich die Herkünfte der Arten, die am Prozess der Lauro- phyllisierung beteiligt sind, für die Verwendung im städtischen Raum eignen und somit beantworten, inwiefern die Laurophyllisierung eine Chance für die klimaresiliente Gehölzverwendung in der Stadt dar- stellen kann.
Dafür werden die unterschiedlichen Baumstandorte einer Stadt anhand thermischer und hygrischer Verhältnisse zu schematischen Baumstandorttypen zusammengefasst. Während sich durch die ther- mischen Verhältnisse vor allem zentral und dezentral gelegene Baumstandorttypen differenzieren las- sen, trennen die hygrischen Verhältnisse naturnahe Baumstandorttypen von Baumstandorttypen mit eingeschränktem Wurzelraum. Für diese vier Baumstandorttypen wird dann für vier unterschiedliche Städte Deutschlands das zukünftige Mikroklima mithilfe des RCP8.5 in Form eines Klimadiagramms er- mittelt. Durch die differenzierte Auswahl der Untersuchungsgebiete Hamburg, Düsseldorf, München und Berlin können so Aussagen zu regionalen Auswirkungen des Klimawandels getroffen werden. Zudem dienen die Klimadiagramme der unterschiedlichen Baumstandorttypen als Grundlage für den klimati- schen Vergleich mit den Klimaten der Herkünfte der immergrünen Arten des Zonobiom V. Anhand die- ses Vergleiches können Subzonobiome ausfindig gemacht werden, die mehr oder weniger dem Klima der Baumstandorttypen entsprechen. Eines der Subzonobiome, welches eine gute klimatische Referenz darstellt, wird anschließend im Hinblick auf die prägnantesten immergrünen Arten näher betrachtet. Die Auswahl in dieser Arbeit ist auf das sommerregengeprägte Subzonobiom Ost-Asiens gefallen, aus dem insgesamt 20 immergrüne Laubbaumarten, neun Koniferen und eine Palmenart in Form von Steck- briefen näher beschrieben und auf die Eignung für die Verwendung innerhalb der Mirkoklimate der vier Baumstandorttypen eingeschätzt werden.
In dieser Arbeit soll an der Schnittstelle zwischen nachhaltiger Ernährung und nachhaltiger Landwirtschaft ein konkreter Planungsvorschlag eines AFS zur Anlage an der Hochschule (HS) Osnabrück entworfen werden. Auf diese Weise soll aufgezeigt werden, wie Agroforstsysteme zur Realisierung der Ziele der Planetary Health Diet beitragen können. Das so entstehende AFS soll zukünftig auch zu einem ernährungswissenschaftlichen Ort des Lehrens und
Lernens weiterentwickelt werden können – zum Beispiel einem Lehr- und Lerngarten. Auf diese Weise soll eine nachhaltige Ernährungsweise, die PHD in einem zukunftsweisenden Anbausystem, dem AFS pflanzenbaulich dargestellt werden. Hierzu sollen auch Standortfaktoren und Betriebsspiegel des Versuchsbetriebes der Hochschule geschickt einbezogen werden.
Diet can influence healthy aging through anti- or proinflammatory effects, partly by modulating the gut microbiome composition. This study investigated the relationships between the Dietary Inflammatory Index (DII), the gut microbiome, and nutritional status in elderly individuals. Methods: This cross-sectional analysis included 114 home-dwelling individuals aged over 70 years. The Energy-adjusted DII (E-DII) was calculated from 3-day food diaries, and blood samples were taken to measure micronutrient status, glucose, and lipid metabolism. Body composition was assessed using bioimpedance, and fecal gut microbiome composition was analyzed through 16S rRNA gene sequencing. The participants were categorized into maintaining an anti-inflammatory diet (AD) and a pro-inflammatory diet (PD) based on the median E-DII score. The associations of E-DII groups with blood markers and microbial diversity and composition were examined using the analysis of covariance, permutational analysis of variance, and multivariate linear models. Results: The AD (n = 57, 76 ± 3.83 years) and PD (n = 57, 75 ± 5.21 years) groups were similar in age but differed in sex distribution, with a higher proportion of females in the AD group (p = 0.02). When compared to the PD group and adjusted for sex, the AD group had a lower body mass index, fat mass, fasting insulin level, HOMA-IR (Homeostasis Model Assessment of Insulin Resistance), fasting triglycerides, and serum uric acid concentration (all p < 0.05), with higher concentrations of high-density lipoprotein, red-blood-cell folate (RBC), and Omega-3 index (all p < 0.05). While the microbial diversity and composition did not differ between the DII groups, folate concentrations were negatively associated with Agathobacter and positively associated with Bacteroides abundance (both q = 0.23). Lower uric acid concentrations were associated with a higher abundance of Bifidobacterium (q = 0.09) and lower abundance of Phocaeicola (q = 0.11). Discussion: The study suggests that following an anti-inflammatory diet is associated with improved nutritional status in the elderly. Dietary blood markers, rather than E-DII, were found to be associated with the gut microbiome, suggesting a potential link between the microbiome and changes in nutritional markers independent of diet. Further studies are needed to explore the causal relationship between dietary inflammatory potential, gut microbiome, and healthy aging.
Dissertation zur Erlangung des Doktorgrades (Dr. rer. nat.)
Universität Osnabrück
Fachbereich Kultur- und Sozialwissenschaften
Institut für Geographie
in Kooperation mit der Hochschule Osnabrück
Fakultät Agrarwissenschaften und Landschaftsarchitektur
In a protein reduction feeding trial (Study 1) on a commercial broiler farm in northern Germany, it was attempted to be shown that research results from station tests on protein reduction can be transferred to agricultural practice. In a second study, the limits of the N reduction were tested in a research facility. In Study 1, commercial standard feeds were fed to the control group (variant 1:210,000 animals; n = 5 barns). In the test group (variant 2:210,000 animals; n = 5 barns), the weighted mean crude protein (CP) content was moderately reduced by 0.3%. The nitrogen reduction in the feed did not affect performance (feed intake (FA), daily gain (DG), feed conversion (FCR)), but nitrogen conversion rate increased from approx. 61% to approx. 63%. The solid litter weight was reduced by 12% and nitrogen excretion by 9% (p < 0.05). Significantly healthier footpads were due to lower water intake (−4%; p < 0.05) and a numerically drier bedding. In Study 2, responses of treatments (1250 broiler per variant; n = 5) showed that sharper N-lowering (−1.5% CP; weighted average) did not impair performance either, but N-conversion improved and N-excretions decreased significantly. Converted to a protein reduction of one percentage point, the N excretions were able to be reduced by 22% in Study 1 and 18% in Study 2. Feeding trials in the commercial sector, such as the present Study 1, should convince feed mills and farmers to allow the latest scientific results to be used directly and comprehensively in commercial ration design.
Duckweed is gaining attention in animal nutrition and is considered as a potential alternative protein source for broiler chickens. In order to evaluate the nutritional value of duckweed, three individual batches were investigated. They consisted of a mixture of Lemna minuta and Lemna minor (A, 17.5% crude protein), Spirodela polyrhiza (B, 24.6% crude protein) and Lemna obscura (C, 37.0% crude protein). Treatment diets contained 50% batch A, 50% batch B, and 25, 50 and 75% of batch C. All diets were fed to broiler chickens (Ross 308) from an age of 21 to 27 days. Diets with a share of 50 and 75% of batch C led to decreased feed intake (109.3 and 74.9 g/day, respectively) compared to the control. Standardized ileal digestibility of crude protein and amino acids differed significantly between duckweed batches, at values for methionine between 49.9 and 90.4%. For all amino acids, batch A consistently had the lowest and batch C the highest digestibility. Batches had different tannin contents of 2943, 2890 and 303 mg/kg for batches A, B and C, respectively. The apparent ileal digestibility of phosphorus differed significantly between all batches (50.8–78.9%). Duckweed can be used as a protein feed for broiler chickens. However, a defined and stable biomass composition optimized for the requirements of broiler chickens is needed.
Hintergrund
Die Anämie hat eine hohe Prävalenz bei Patienten vor Hüftgelenkrevisionsoperation und ist mit einer erhöhten Komplikationsrate assoziiert. Die vorliegende Arbeit untersucht erstmals den Zusammenhang von Kosten, realen DRG-Erlösen und Falldeckung der präoperativen Anämie bei elektiven Hüftgelenkrevisionsoperationen.
Methoden
Für alle Patienten, die sich von 2010 bis 2017 an 2 Campi der Charité – Universitätsmedizin Berlin einer Hüftgelenkrevisionsoperation unterzogen, wurden Daten zu Patienten sowie Transfusionen, Kosten und Erlösen gesammelt. Subgruppen- und lineare Regressionsanalysen untersuchten die Falldeckung anämischer und nichtanämischer Patienten.
Ergebnisse
Von 1187 eingeschlossenen Patienten waren 354 (29,8 %) präoperativ anämisch. Insgesamt wurden 565 (47,6 %) Patienten, mit einem deutlichen Überwiegen anämischer Patienten (72,6 % vs. 37,0 %, p < 0,001), transfundiert. Kosten (12.318 € [9027;20.044 €] vs. 8948 € [7501;11.339 €], p < 0,001) und Erlöse (11.788 € [8992;16.298 €] vs. 9611 € [8332;10.719 €], p < 0,001) waren für anämische Patienten höher, die Fallkostendeckung defizitär (−1170 € [−4467;1238 €] vs. 591 € [−1441;2103 €] €, p < 0,001). Bei anämischen Patienten nahm die Falldeckung mit zunehmender Transfusionsrate ab (p ≤ 0,001). Komorbiditäten hatten keinen signifikanten ökonomischen Einfluss.
Schlussfolgerung
Die präoperative Anämie und perioperative Transfusionen bei Hüftgelenkrevisionsoperationen sind mit erhöhten Behandlungskosten und einer finanziellen Unterdeckung für Kostenträger im Gesundheitswesen verbunden. Konzepte zur Behandlung der präoperativen Anämie (z. B. Patient Blood Management) könnten mittelfristig Behandlungskosten senken.
Easy and inexpensive methods for measuring ammonia emissions in multi-plot field trials allow the comparison of several treatments with liquid manure application. One approach that might be suitable under these conditions is the dynamic tube method (DTM). Applying the DTM, a mobile chamber system is placed on the soil surface, and the air volume within is exchanged at a constant rate for approx. 90 s. with an automated pump. This procedure is assumed to achieve an equilibrium ammonia concentration within the system. Subsequently, a measurement is performed using an ammonia-sensitive detector tube. Ammonia fluxes are calculated based on an empirical model that also takes into account the background ammonia concentration measured on unfertilized control plots. Between measurements on different plots, the chamber system is flushed with ambient air and cleaned with paper towels to minimize contamination with ammonia. The aim of this study was to determine important prerequisites and boundary conditions for the application of the DTM.
We conducted a laboratory experiment to test if the ammonia concentration remains stable while performing a measurement. Furthermore, we investigated the cleaning procedure and the effect of potential ammonia carryover on cumulated emissions under field conditions following liquid manure application. The laboratory experiment indicated that the premeasurement phase to ensure a constant ammonia concentration is not sufficient. The concentration only stabilized after performing more than 100 pump strokes, with 20 pump strokes (lasting approximately 90 s) being the recommendation.
However, the duration of performing a measurement can vary substantially, and linear conversion accounts for those differences, so a stable concentration is mandatory. Further experiments showed that the cleaning procedure is not sufficient under field conditions. Thirty minutes after performing measurements on high emitting plots, which resulted in an ammonia concentration of approx.
10 ppm in the chamber, we detected a residual concentration of 2 ppm. This contamination may affect measurements on plots with liquid manure application as well as on untreated control plots. In a field experiment with trailing hose application of liquid manure, we subsequently demonstrated that the calculation of cumulative ammonia emissions can vary by a factor of three, depending on the degree of chamber system contamination when measuring control plots. When the ammoni background values were determined by an uncontaminated chamber system that was used to measure only control plots, cumulative ammonia emissions were approximately 9 kg NH3-N ha1.
However, when ammonia background values were determined using the contaminated chamber system that was also used to measure on plots with liquid manure application, the calculation of cumulative ammonia losses indicated approximately 3 kg NH3-N ha1. Based on these results, it can be concluded that a new empirical DTM calibration is needed for multi-plot field experiments with high-emitting treatments.
The BBI is a first step toward putting biodiversity conservation into practice in the OHC context. The results are consistent with studies related to nutrition However, the results also show that there is room for improvement and that there are further areas to be addressed. It is also clear that commercial kitchens currently have only limited room for maneuver. If OHC is to become more biodiversity-friendly, greater transparency is needed in terms of origin labels and species/variety identifiers, and a wide range of options will also be required in terms of procurement. That being the case, it is essential to focus on the entire value chain. Furthermore, in addition to the initial recommendations, much more knowledge is required about the impacts of farming methods and heritage varieties and species, as well as about the use of fish, other marine animals and game meat. In principle, however, the BBI can already be implemented in commercial kitchens by identifying recipe optimizations that kitchens can feasibly implement, that align with their budgets, and that maintain acceptance among patrons. In addition, this approach has the potential to be integrated into the assessment framework of the NAHGAST calculator, making it readily accessible and free for OHC facilities to use. In the OHC context in particular, this could be leveraged to drive sustainable change in the food system.
Plant-based proteins are rapidly emerging, while novel technologies are explored to offer more efficient extraction processes. The current study aimed to evaluate the effects of pulsed electric fields (PEFs) and temperature on the extraction of soluble proteins from nettle leaves (Urtica dioica L.) and identify an optimal operational range for the highest yield of soluble proteins. Extractions and kinetic modeling were conducted with whole and ground dried leaves at different temperatures (30–70 °C) and specific energy of PEF (0–30 kJ kg−1) with extraction times of up to 60 min. The influence of temperature and specific energy on the soluble protein extraction yields was investigated and modeled using composite central design and response surface methodology. The experimental results were fitted to Peleg's kinetic model, which satisfactorily described the extraction process (R2 > 0.902), and PEF treated samples resulted in a higher soluble protein yield and shortened processing time. Response surface methodology showed that the linear effect of temperature and quadratic effect of PEF (p < 0.01) were highly significant for protein yield. In the optimized PEF-extraction region (specific energy between 10 and 24 kJ kg−1, and 70–78 °C), soluble protein yield was higher than 60% after 5 minutes of extraction. The achieved results are relevant for developing processes for PEF assisted extraction of soluble proteins from leaves. Understanding the effects of PEFs and process parameters is crucial to obtain high protein yields, while requiring low energy and short processing time.
The kiwifruit processing industry is focused on product yield maximization and keeping energy costs and waste effluents to a minimum while maintaining high product quality. In our study, pulsed electric field (PEF) pretreatment enhanced kiwifruit processing to facilitate peelability and specific peeling process and enhanced valorization of kiwifruit waste. PEF optimization was applied to obtain the best treatment parameters. A 32 factorial design of response surface methodology was applied to find the effect of time elapsed after PEF treatment and the PEF-specific energy input on specific peeling force and kiwifruit firmness as response criteria. Under the optimized condition, the specific peeling force decreased by 100, and peelability increased by 2 times. The phenolic content and antioxidant capacity of PEF-treated kiwifruit bagasse were 5.1% and 260% richer than the control sample. Overall, the optimized PEF pretreatments incorporated into kiwifruit processing led to decreased energy demand and increased productivity.
Der Text behandelt die Bedeutung nachhaltiger Ernährung und die Herausforderungen bei der Integration von Nachhaltigkeitsaspekten in die berufliche Bildung, insbesondere in den Korn-handhabenden Berufen. Tägliche Ernährungsentscheidungen werden als maßgeblich für Umwelt, Gesellschaft und Gesundheit hervorgehoben. Die EAT-Lancet-Kommission schlägt eine Planetarische Gesundheitsdiät vor, die klimafreundlich und gesund ist. Die Förderung von Nachhaltigkeitskompetenzen in der beruflichen Bildung wird als Schlüssel für langfristige Veränderungen in Betrieben betont. Der Modellversuch Korn-Scout zielt darauf ab, Lehr-/Lernmaterialien zu entwickeln, die die Nachhaltigkeitsorientierung von Auszubildenden in Korn-handhabenden Berufen fördern. Dieser besteht aus vier Elementen: Status-quo-Analyse, Entwicklung von Lehr-/Lernmaterialien, Erprobung und Transfer in die Praxis. Kommunikation wird als Schlüsselfaktor für die systematische Integration von Bildung für nachhaltige Entwicklung in die Ausbildung betont. Die Evaluation der Materialien während der Covid-19-Pandemie führte zu sinnvollen Optimierungsvorschlägen.Erunterstreicht die Bedeutung von Kommunikationskompetenzen für nachhaltiges Handeln in den Korn-handhabenden Berufen. Effektive Kommunikation entlang der Wertschöpfungskette ist entscheidend, um Nachhaltigkeitsaspekte von der landwirtschaftlichen Produktion bis zum Verzehr zu integrieren. Neben der Kommunikationskompetenz werden auch andere Fähigkeiten wie die Suche, Verarbeitung und Integration von nachhaltigkeitsrelevanten Informationen betont. Der Modellversuch Korn-Scout identifizierte berufsübergreifende Nachhaltigkeitskompetenzen entlang der Korn-Food-Supply-Chain, wobei einige Berufe spezifische Kompetenzen aufweisen. Die systematische Weiterentwicklung dieser Kompetenzen ist entscheidend für die strukturelle Integration von Nachhaltigkeit in der beruflichen Praxis. Lehr-/Lernmaterialien aus dem Modellversuch werden durch Zusammenarbeit mit Praxispartnern in die Praxis übertragen. Der Ausblick betont die Notwendigkeit weiterer Projekte und Anpassungen in den Ausbildungsordnungen für eine umfassende Verankerung von Nachhaltigkeit in der beruflichen Ausbildung.
Ohmic heating (OH) is an alternative sustainable heating technology that has demonstrated its potential to modify protein structures and aggregates. Furthermore, certain protein aggregates, namely amyloid fibrils (AF), are associated with an enhanced protein functionality, such as gelation. This study evaluates how Ohmic heating (OH) influences the formation of AF structures from ovalbumin source under two electric field strength levels, 8.5 to 10.5 and 24.0–31.0 V/cm, respectively. Hence, AF aggregate formation was assessed over holding times ranging from 30 to 1200 sunder various environmental conditions (3.45 and 67.95 mM NaCl, 80, 85 and 90 °C, pH = 7). AF were formed under all conditions. SDS-PAGE revealed that OH had a higher tendency to preserve native ovalbumin molecules. Furthermore, Congo Red and Thioflavin T stainings indicated that OH reduces the amount of AF structures. This finding was supported by FTIR measurements, which showed OH samples to contain lower amounts of beta-sheets. Field flow fractioning revealed smaller-sized aggregates or aggregate clusters occurred after OH treatment. In contrast, prolonged holding time or higher treatment temperatures increased ThT fluorescence, beta-sheet structures and aggregate as well as cluster sizes. Ionic strength was found to dominate the effects of electric field strength under different environmental conditions.
Olive oil holds significant importance in the European diet and is renowned globally for its sensory attributes and health benefits. The effectiveness of producing olive oil is greatly influenced by factors like the maturity and type of olives used, as well as the milling techniques employed. Generally, mechanical methods can extract approximately 80% of the oil contained in the olives. The rest 20% of the oil remains in the olive waste generated at the end of the process. Additionally, significant amounts of bioactive compounds like polyphenols are also lost in the olive pomace. Traditionally, heat treatment, enzymes, and other chemicals are used for the enhancement of oil extraction; however, this approach may impact the quality of olive oil. Therefore, new technology, such as pulsed electric field (PEF), is of great benefit for nonthermal yield and quality improvements.
Die Autoren präsentieren einen innovativen Ansatz für Circular Economy (CE)-Strategien mit hohem Umweltpotenzial. Dieser Ansatz betont Verweigerung, Umdenken und Reduzieren, indem die Perspektive vom Verbraucher auf soziale Praktiken verschoben wird. Dabei wird Suffizienz als Schlüsselkonzept eingeführt, das entscheidend ist, um Umweltbelastungen durch CE zu reduzieren und die Transformation des Produktions- und Konsumsystems zu fördern. Die Autoren betonen, dass nachhaltige Kreislaufwirtschaft ohne Suffizienz nicht möglich ist.
Die Studie berichtet über einen Fall, in dem die Über-Verfügbarkeit durch das Konzept der Genügsamkeit ersetzt wurde. Die Autoren beobachten, wie Suffizienz in die Lebensstile der Teilnehmer integriert wird, insbesondere durch einen Farmboxing-Ansatz. Obwohl die Verallgemeinerung des Falls begrenzt ist, zeigt die Studie das Potenzial niedrigschwelliger Einführungen von Suffizienz, wie beispielsweise öffentlicher Raum für gärtnerische Aktivitäten.
Die Autoren betonen jedoch die Notwendigkeit weiterer empirischer Arbeit, um die Bedeutung von Suffizienz in verschiedenen sozialen Praktiken zu verstehen. Sie schlagen vor, dass der Zeitpunkt für solche Forschungen günstig ist, angesichts aktueller globaler Entwicklungen und der steigenden Nachfrage nach Strategien zur Ressourcenverringerung.
Die Studie identifiziert Herausforderungen, darunter unklare Faktoren, die nicht berücksichtigt wurden, wie die Rolle des sozialen Umfelds und die Übertragbarkeit der Ergebnisse auf andere Lebensbereiche. Die Autoren planen bereits eine Folgestudie mit quantitativen und qualitativen Analysen sowie Lebenszyklusanalysen.
Abschließend schlagen die Autoren vor, klare Definitionen für Wiederverwendung, Umdenken und Reduzierung zu entwickeln, basierend auf empirischen Daten und Beispielen sozialer Praktiken. Sie fordern auch dazu auf, Theorien sozialer Praktiken auf andere CE-Strategien anzuwenden, da soziale Praktiken nicht auf privaten Konsum beschränkt sind.
Hintergrund
Die Anämie hat eine hohe Prävalenz bei Patienten vor Hüftgelenkrevisionsoperation und ist mit einer erhöhten Komplikationsrate assoziiert. Die vorliegende Arbeit untersucht erstmals den Zusammenhang von Kosten, realen DRG-Erlösen und Falldeckung der präoperativen Anämie bei elektiven Hüftgelenkrevisionsoperationen.
Methoden
Für alle Patienten, die sich von 2010 bis 2017 an 2 Campi der Charité – Universitätsmedizin Berlin einer Hüftgelenkrevisionsoperation unterzogen, wurden Daten zu Patienten sowie Transfusionen, Kosten und Erlösen gesammelt. Subgruppen- und lineare Regressionsanalysen untersuchten die Falldeckung anämischer und nichtanämischer Patienten.
Ergebnisse
Von 1187 eingeschlossenen Patienten waren 354 (29,8 %) präoperativ anämisch. Insgesamt wurden 565 (47,6 %) Patienten, mit einem deutlichen Überwiegen anämischer Patienten (72,6 % vs. 37,0 %, p < 0,001), transfundiert. Kosten (12.318 € [9027;20.044 €] vs. 8948 € [7501;11.339 €], p < 0,001) und Erlöse (11.788 € [8992;16.298 €] vs. 9611 € [8332;10.719 €], p < 0,001) waren für anämische Patienten höher, die Fallkostendeckung defizitär (−1170 € [−4467;1238 €] vs. 591 € [−1441;2103 €] €, p < 0,001). Bei anämischen Patienten nahm die Falldeckung mit zunehmender Transfusionsrate ab (p ≤ 0,001). Komorbiditäten hatten keinen signifikanten ökonomischen Einfluss.
Schlussfolgerung
Die präoperative Anämie und perioperative Transfusionen bei Hüftgelenkrevisionsoperationen sind mit erhöhten Behandlungskosten und einer finanziellen Unterdeckung für Kostenträger im Gesundheitswesen verbunden. Konzepte zur Behandlung der präoperativen Anämie (z. B. Patient Blood Management) könnten mittelfristig Behandlungskosten senken.
Introduction Postoperative delirium (POD) is seen in approximately 15% of elderly patients and is related to poorer outcomes. In 2017, the Federal Joint Committee (Gemeinsamer Bundesausschuss) introduced a ‘quality contract’ (QC) as a new instrument to improve healthcare in Germany. One of the four areas for improvement of in-patient care is the ‘Prevention of POD in the care of elderly patients’ (QC-POD), as a means to reduce the risk of developing POD and its complications.
The Institute for Quality Assurance and Transparency in Health Care identified gaps in the in-patient care of elderly patients related to the prevention, screening and treatment of POD, as required by consensus-based and evidence-based delirium guidelines. This paper introduces the QC-POD protocol, which aims to implement these guidelines into the clinical routine. There is an urgent need for well-structured, standardised and interdisciplinary pathways that enable the reliable screening and treatment of POD. Along with effective preventive measures, these concepts have a considerable potential to improve the care of elderly patients.
Methods and analysis The QC-POD study is a non-randomised, pre–post, monocentric, prospective trial with an interventional concept following a baseline control period. The QC-POD trial was initiated on 1 April 2020 between Charité-Universitätsmedizin Berlin and the German health insurance company BARMER and will end on 30 June 2023. Inclusion criteria: patients 70 years of age or older that are scheduled for a surgical procedure requiring anaesthesia and insurance with the QC partner (BARMER). Exclusion criteria included patients with a language barrier, moribund patients and those unwilling or unable to provide informed consent. The QC-POD protocol provides perioperative intervention at least two times per day, with delirium screening and non-pharmacological preventive measures.
Ethics and dissemination This protocol was approved by the ethics committee of the Charité-Universitätsmedizin, Berlin, Germany (EA1/054/20). The results will be published in a peer-reviewed scientific journal and presented at national and international conferences.
A brief questionnaire for measuring alarm fatigue in nurses and physicians in intensive care units
(2023)
When exposed to hundreds of medical device alarms per day, intensive care unit (ICU) staff can develop “alarm fatigue” (i.e., desensitisation to alarms). However, no standardised way of quantifying alarm fatigue exists. We aimed to develop a brief questionnaire for measuring alarm fatigue in nurses and physicians. After developing a list of initial items based on a literature review, we conducted 15 cognitive interviews with the target group (13 nurses and two physicians) to ensure that the items are face valid and comprehensible. We then asked 32 experts on alarm fatigue to judge whether the items are suited for measuring alarm fatigue. The resulting 27 items were sent to nurses and physicians from 15 ICUs of a large German hospital. We used exploratory factor analysis to further reduce the number of items and to identify scales. A total of 585 submissions from 707 participants could be analysed (of which 14% were physicians and 64% were nurses). The simple structure of a two-factor model was achieved within three rounds. The final questionnaire (called Charité Alarm Fatigue Questionnaire; CAFQa) consists of nine items along two scales (i.e., the “alarm stress scale” and the “alarm coping scale”). The CAFQa is a brief questionnaire that allows clinical alarm researchers to quantify the alarm fatigue of nurses and physicians. It should not take more than five minutes to administer.
ZUKUNFTSBÄUME FÜR MÜNSTER – ERARBEITUNG EINES BAUMENTWICKLUNGSKONZEPTES MIT STANDORTSPEZIFISCHEN ARTENEMPFEHLUNGEN UNTER ZUKÜNFTIGEN KLIMABEDINGUNGEN UNTER BERÜCKSICHTIGUNG DES KLIMAHÜLLENKONZEPTES
Durch den voranschreitenden Klimawandel werden die ohnehin ungünstigen Standortbedingungen von Bäumen im urbanen Raum zunehmend schwieriger. Gleichzeitig steigt die Bedeutung von Stadtbäumen, denn im Rahmen der Klimaanpassung haben ihre Ökosystemleistungen einen besonders hohen Stellenwert. Viele der gängigen Arten leiden allerdings stark unter den bereits spürbaren Auswirkungen des Klimawandels – auch im klimatisch vergleichsweise milden Münster. In der vorliegenden Arbeit wird eine vereinfachte Typologisierung der urbanen Baumstandorte in Münster vorgenommen. Unter Berücksichtigung der stadtklimatischen Besonderheiten und spezifischen Einschränkungen wird das Klimahüllenkonzept von Kölling auf die jeweiligen Standorttypen übertragen und in Bezug zu den zukünftig erwarteten klimatischen Verhältnissen gesetzt. Hierbei zeigt sich, dass zum Ende des aktuellen Jahrhunderts nicht mehr viele der klassischen Arten als Stadtbäume in Münster geeignet sein werden. Daher werden standortbezogene Zukunftsbaum-Empfehlungen in Form eines vereinfachten Baumentwicklungskonzeptes erarbeitet. Die Standortgerechtigkeit und die Diversität des urbanen Baumbestandes werden im Hinblick auf die geänderten klimatischen Bedingungen in Zukunft enorm an Bedeutung gewinnen. Die Erweiterung und Optimierung des zukünftigen Stadtbaumsortiments ist insofern ein wichtiger Baustein zur Anpassung des urbanen Baumbestandes an den voranschreitenden Klimawandel.
Im Rahmen der vorliegenden Masterarbeit im Studiengang Landschaftsarchitektur an der Hochschule Osnabrück wurden Untersuchungen zur Winteraktivität der Zwergfledermaus (Pipistrellus pipistrellus) in Osnabrück (Niedersachsen) durchgeführt und Methoden zum ultraschallakustischen Nachweis von Gebäudequartieren erprobt und weiterentwickelt. Vorausgegangen war eine Online-Umfrage bei 17 Mitarbeitenden Unterer Naturschutzbehörden in Niedersachsen zur Verfahrenspraxis bei fledermauskritischen Gebäudesanierungen und Abrissen. Defizite bestehen demnach möglicherweise nicht nur in der Qualität fledermauskundlicher Erfassungen, sondern auch in einer gravierenden Kenntnislücke der zuständigen Behörden über Vorhaben, bei denen gebäudebewohnende Fledermäuse und ihre Quartiere beeinträchtigt werden. Das Feststellen von schwärmenden Zwergfledermäusen kann dazu dienen, noch während der Überwinterung eine Quartiersnutzung festzustellen. Winterschwärmkontrollen sollten daher in allgemeine Methodenempfehlungen Eingang finden, um bei Artenschutzprüfungen eine bessere Wissensgrundlage über die phänologische Quartiersfunktion und -nutzung zu ermöglichen.
Akustische Quartiersnachweise können sowohl über aktive Untersuchungen als auch unter bestimmten Voraussetzungen mithilfe passiver automatischer Ultraschallerfassungen erfolgen. Optische Hilfsmittel, vorzugsweise moderne Wärmebildkameras, sollten darüber hinaus unbedingt hinzugezogen werden. In der Stadt Osnabrück konnten in einer mehrtägigen Frostperiode Ende Dezember 2021 vier neue Winterquartiere der Zwergfledermaus bei Begehungen mit Detektor gefunden werden. Ein parallel durchgeführtes Dauermonitoring an einem großen Winterquartier über den Zeitraum 29.10.2021 bis 01.03.2022 offenbarte erhöhte Schwärmaktivität nicht nur bei Frost, sondern ebenfalls nach kräftigen Temperaturanstiegen mit Nachttemperaturen bis über 10°C. Die genauen Zusammenhänge zwischen allgemeiner Aktivität und Witterungsbedingungen sind komplex und benötigen weitergehender Untersuchungen. Auch auf Grundlage des derzeitigen Kenntnisstandes können großräumige Quartierssuchen in Form von winterlichen Schwärmkontrollen bereits jetzt dazu beitragen, essentielle Quartiere der Zwergfledermaus zu identifizieren. Den Unteren Naturschutzbehörden wird u.a. vorgeschlagen, ein Kataster über diese Quartiere in ihrem Zuständigkeitsbereich zu führen und beständig zu aktualisieren.
Background
The aim of this qualitative study was to identify a practice level model that could explain a sustained change in nutritional behavior.
Methods
The study used three data inputs from four interviewees, one merged input from a married couple, as narrative interviews. The interviews were analyzed using grounded theory.
Results
Coexistence of a certain suffering and a triggering episode lead to the decision to change nutritional life-style by all interviewed. Maintenance of the self-determined newly learned nutritional behavior was supported by subject-related intrinsic motivation, the ability to reflect, and a low expectation of success from the behavioral change. Environment-related factors were identified as support from life-partner and peers. Subjects reported that the sustained nutritional behavior change impacted their holistic health through subject-perceived improved life quality, increase in the number of social contacts, and a change in personal attitudes and perception. The analysis remains limited, and at best hypothesis generating, in that only three data inputs from four interviewees were used.
Conclusion
In this hypothesis-generating narrative interview study of four study subjects, volition, personal decision making, and long-term motivation (though not external determination) seemed to sustain a change in newly learned nutritional behavior.
Adventitious root (AR) formation is the basis of vegetative propagation in rose, be it via stem cuttings or via stenting. During this process, wounding plays a pivotal role since cell reprogramming takes place at the tissue adjacent to the wound. We investigated the effects of wounding on AR formation on leafy single-node stem cuttings of the rose rootstock R. canina ‘Pfänder’ (codes R02-3 and R02-6) and the cut rose cultivar Rosa ‘Tan09283’ (Registration name ‘Beluga’). Laser wounding treatments were based on the assisted removal of tissue layers located in the bark. The positioning of wounding was studied based on two marking directions: along the cutting base (strip pattern) and around the cutting base (ring pattern). Additionally, the effects of external supply of indole-butyric acid (IBA 1 mg L-1) on rootingwere analyzed. Results showedthat inorder toremovespecific tissue layers, the calculation of the laser energy density (J cm-2) in terms of cutting diameter was necessary. Interestingly, the application of energy densities from 2.5 J cm-2 up to approximately 8.5 J cm-2 were sufficient to expose the tissue layers of epidermis up to regions of phloem. Regarding AR formation for R. canina ‘Pfänder’, characterized by a low rooting response, an increase in the rooting percentage was registered when the laser treatment eliminated the tissue up to phloem proximities. Analysis of the nodal position showed that bud location was a preferential place for AR formation independently of wounding treatment. In case of Rosa ‘Tan09283’, laser treatments did not reduce its high rooting capacity, but an apparent reduction in rooting quality due to an investment in tissue healing was observed when wounding reached deeper layers such as parenchyma and sclerenchyma. Results also showeda strongARformation directly fromwounded regions in case of Rosa ‘Tan09283’ specifically when the woundwas located below the axillary bud. In conclusion, wounding by assisted-elimination of layers by laser can induce positive effects on AR formation of single-node stemcuttings of the rose if energy applied is able to expose phloemproximities,a longitudinalorientation, and relative position to the axillary bud are considered.
Die Innenentwicklung ist ein wichtiger Baustein für eine nachhaltige Stadtentwicklung. Jedoch entstehen in der praktischen Umsetzung häufig Zielkonflikte zwischen verschiedenen Themen der nachhaltigen Stadtentwicklung. Die in § 1 BauGB aufgeführten Ziele und Belange müssen in der Bauleitplanung auf kommunaler Ebene im Rahmen der Abwägung berücksichtigt und gewichtet werden. Dies kann für kommunale Akteur*innen eine große Herausforderung darstellen.
Im Rahmen des Forschungsprojekts „Rechtsanwendung in der Bauleitplanung – Umgang mit Zielkonflikten im Bereich der Innenentwicklung“ (gefördert aus Mitteln des Niedersächsischen Vorab) wurde untersucht, welche Zielkonflikte in der verbindlichen Bauleitplanung benannt werden und welche Lösungsansätze dazu von Kommunen und in der Forschung entwickelt wurden. Die Erkenntnisse basieren unter anderem auf einer umfangreichen Dokumentenanalyse von über 80 Bebauungsplänen deutscher wachsender Großstädte.
Die vorliegende Arbeitshilfe zeigt im Sinne eines Baukastensystems 49 ausgewählte Bausteine aus sechs Handlungsfeldern der Innenentwicklung. Damit sollen Kommunen Hinweise an die Hand gegeben werden, wie in der verbindlichen Bauleitplanung Zielkonflikte zwischen verschiedenen Handlungsfeldern bewältigt und Lösungsansätze anhand der Festsetzungen nach § 9 BauGB planungsrechtlich im Bebauungsplan verankert werden können.
The production of food-grade substances and complex biocatalysts used as additives or active ingredients – mainly for food applications – can be achieved in the eukaryotic expression system of Aspergillus niger. Food proteins or food enzymes e.g., casein, ovalbumin, phytase or glucoamylase are highly complex polymers. Most of them could be used as nitrogen or energy source for animals and humans, while others are industrial relevant biomass-degrading enzymes used for biological waste processing and food production.
However, the successful production of novel recombinant proteins can be challenging, resource- and time consuming. Therefore, A. niger mutant libraries are needed to understand the “adjusting screws” to produce high yields of recombinant proteins, preferably even in a kind of generic, transferable system. In order to establish a universal and multipurpose expression platform, there is the need to overcome the lack of high throughput assays first.
To tackle this problem, we designed a modular, quantitative and feasible high-throughput screening system to express and screen recombinant proteins regarding their stability and functionality in A. niger. For this purpose a dual-luciferase reporter gene system, which is applicable in small scale will be established for A. niger. After the generation of an A. niger secretion mutant library, the system will be transferred and tested to other proteins of interest. The technology can be integrated into bio-regenerative life support systems for the autonomous production of e.g., food, food additives and food enzymes on earth as well as in deep-space.
Der Ökologische Landbau hat als Ziel die Biodiversität, die vor allem durch die Flora beeinflusst wird, zu stärken. In Verbindung mit dem Ertragsziel der landwirtschaftlichen Produktion ergibt sich dadurch die Frage, mit welchen Maßnahmen sowohl eine höhere Beikrautbiodiversität als auch die Ertragssicherung kombiniert werden können.
Hierzu wurde in der Nähe von Osnabrück ein einjähriger Versuch mit ökologisch angebauter Wintertriticale angelegt. Der Versuch wurde als Split-Plot-Design mit 4 Blöcken und Variationen von Saattermin, Saatstärke und mechanischer Beikrautregulierung an zwei Terminen im Frühjahr, auch mit Hackvarianten in 25 cm Reihenabstand, angelegt und von März 2022 bis zur Ernte im Juli 2022 regelmäßig auf vegetative und später auch generative Triticaleparameter sowie auf das Auftreten verschiedener Beikrautarten und den Beikrautdeckungsgrad geprüft.
Durch den frühen Saattermin am 18.10.2021 konnte verglichen mit dem späten Saattermin am 01.11.2021 aufgrund der längeren Wachstumsphase ein erhöhtes vegetatives Wachstum der Triticale erreicht werden. Ebenso wurde die Zahl der Beikrautindividuen und -arten gefördert, da sich insbesondere Herbstkeimer durch die zeitige Aussaat besser etablieren konnten. Eine erhöhte Konkurrenzwirkung konnte aufgrund von indifferentem Beikrautdeckungsgrad und gleichbleibender Beikrautbiomasse nicht festgestellt werden. Der Kornertrag wurde nicht beeinflusst, aber der Proteingehalt stieg um etwa 0,5 Prozentpunkte an. Entsprechend kann geschlussfolgert werden, dass die Beikrautbiodiversität durch die frühere Aussaat gefördert wurde, während der Strohertrag stieg, der Kornertrag gleichblieb und der Proteingehalt marginal sank.
Die erhöhte Saatstärke führte wie zu erwarten zu einer höheren Bestandesdichte, die auch zu einem höheren Kulturdeckungsgrad führte. Die daraus resultierende höhere Konkurrenzkraft der Triticale führte zu weniger Beikrautbiomasse und weniger Beikrautindividuen sowie -arten. Allerdings konnte in puncto Kornertrag kein Einfluss der Saatstärke festgestellt werden, da die höhere Ährendichte bei höherer Saatstärke durch das Ährengewicht ausgeglichen wurde. Der Proteingehalt stieg sogar durch die reduzierte Saatstärke. Entsprechend kann die reduzierte Aussaatstärke empfohlen werden, da sie positiv für die Beikräuter, indifferent für Stroh- und Kornertrag und positiv für den Proteingehalt eingeschätzt wird. Darüber hinaus können Saatgutkosten eingespart werden.
Den stärksten Einfluss auf Beikräuter und Triticale hatte allerdings die mechanische Beikrautregulierung. Das vegetative und generative Wachstum der einfachen Hackvariante in 25 cm Reihenabstand hat im Vergleich zur Striegelvariante deutliche Vorteile gezeigt. Auch der Proteingehalt und -ertrag konnte erhöht werden. Die intensive Hackvariante mit zusätzlichem Einsatz einer Rotary Hoe verhielt sich intermediär, da sie vermutlich die Kulturpflanze geschädigt hat.
Dagegen wies die Striegelvariante im Vergleich zur intensiven Hackvariante die höchste Zahl an Beikrautarten auf, während allerdings auch Beikrautdeckungsgrad, -biomasse und -individuenzahl maximal waren. Die intensive Hackvariante wies dagegen das ausgeglichenste Beikrautartenverhältnis auf (geringe Dominanz, hohes SDI). Bezüglich der Beikrautparameter verhielt sich die normale Hackvariante intermediär. Bei der normalen Hackvariante konnten entsprechend sämtliche Ertragsparameter maximiert werden, während sich die Beikrautparameter nicht signifikant veränderten. Die Beikrautvielfalt und -abundanz tendierte zu sinken, während die Ausgeglichenheit der Beikrautarten zunahm und entsprechend dominante Arten, wie Acker-Frauenmantel (Aphanes arvensis L.) an Bedeutung verloren haben, was eine Zönose vorbeugt.
Flexibility in planning through frequent amendments. The practice of land use planning in Germany
(2022)
How can formal planning deal with uncertainty and rapid change while maintaining high standards of procedural certainty? This paper, using Germany as an example, examines how municipalities handle this dilemma within their existing planning systems. Municipalities use different approaches, ranging from regular redrafting to comprehensive changes or regular amendments. Amendments and complementary informal plans are important components of flexibility in planning. We argue that the choice to make frequent amendments instead of redrafting the preparatory land-use plan does not necessarily lead to a loss of a city-wide, long-term perspective, provided amendments are prepared and justified using informal concepts.
Taking the transdisciplinary research study “Green fingers for a climate resilient city”, funded by the German Ministry of education and research (BMBF), as an example, this paper follows the hypothesis that processes of landscape planning and designing multifunctional green spaces and processes of co-creation need to be combined to stimulate climate resilient city transformation. The findings are that efforts to combine these processes benefit from making complex climate-resilient city planning accessible for people of different professional backgrounds. The paper showcases how storytelling (Schmidt 2019), mapping (Langner 2009) and guided walks (Schultz 2019) are means to mutually engage with, perceive and understand multifunctional green spaces, inspire ownership, and build capacity for the city’s climate-resilient transformation.
When the ECLAS Conference took place in 1972 western societies were undergoing profound change: They transformed from industrial to postindustrial societies – the so-called service societies. 50 years later, the knowledge society is emerging: Knowledge is considered the key resource of this era. Digitalization and widespread dissemination of ICT allow information to be obtained anywhere anytime. This has severe implications for individual lifestyles and everyday practices. Different aspects of living, learning and working are no longer bound to physical limitations but can be enhanced by or even transferred to the virtual space. So being on the move today means travelling in hybrid spaces. We call this the space and practice “en route”.
At the University of Applied Sciences Osnabrück we explore the following questions:
What does “en route” mean and look like in landscapes of higher education?
How is it perceived individually?
(How) can landscape architecture shape it?
Our transdisciplinary research project EN ROUTE aims to meet current challenges at universities (e.g. digitalisation, sustainable development) with a comprehensive understanding of space and practices “en route”. In a transdisciplinary process, researchers from various disciplines – landscape architecture, geography, urban planning, business administrations and marketing, energy technology and computer science – develop concepts and strategies for sustainable and digital mobility in the higher education sector. New “EN ROUTE” types provide insights into the individual production and utilization of spaces “en route”.
The campuses of the University of Applied Science Osnabrück as well as the virtual and physical space network of its members serve as research example. Initial findings will be presented at the conference. While the ECLAS conference in 1972 focused on physical scales, landscape architecture has to reflect them critically and ask: What could be an innovative understanding of spaces “en route”?
The contribution follows the hypothesis that the concept of transformative resilience can be a driver in transdisciplinary processes bringing together landscape planning and landscape design. Combining processes of generating, structuring and spatializing knowledge on landscape functions and designing visions for sustainable landscapes on different scales benefits from the creative use of mappings.
Duckweeds are fast-growing and nutritious plants, which are gaining increased attention in different fields of application. Especially for animal nutrition, alternative protein sources are needed to substitute soybean meal. The current bottleneck is the standardized production of biomass, which yields stable quantities of a defined product quality. To solve this problem, an indoor vertical farm (IVF) for duckweed biomass production was developed. It consists of nine vertically stacked basins with a total production area of 25.5 m2. The nutrient solution, a modified N-medium, re-circulated within the IVF with a maximum flow rate of 10 L min−1. Nutrients were automatically added based on electrical conductivity. In contrast, ammonium was continuously supplied. A water temperature of 23 °C and a light intensity of 105 μmol m−2 s−1 with a photoperiod of 12:12 h were applied. During a 40-day production phase, a total of 35.6 kg of fresh duckweed biomass (equals 2.1 kg of dried product) was harvested from the IVF. On average, 0.9 kg day−1 of fresh biomass was produced. The dried product contained 32% crude protein (CP) and high levels of proteinogenic amino acids (e.g. lysine: 5.42 g, threonine: 3.85 g and leucine: 7.59 g/100 g CP). Biomass of this quality could be used as a protein feed alternative to soybean meal. The described IVF represents a modular model system for duckweed biomass production in a controlled environment and further innovations and upscaling processes.
25-hydroxycholecalciferol (25-OHD3) formed via hepatic hydroxylation from vitamin D, cholecalciferol, represents the precursor of the biologically active vitamin D hormone, 1,25-dihydroxyvitamin D. Due to a higher absorption rate and the omission of one hydroxylation, dietary supplementation of 25-OHD3 instead of vitamin D3 is considered to be more efficient as plasma concentrations of 25-OHD3 are increased more pronounced. The present review summarises studies investigating potential beneficial effects on mineral homeostasis, bone metabolism, health status and performance in sows, piglets and fattening pigs. Results are inconsistent. While most studies could not demonstrate any or only a slight impact of partial or total replacement of vitamin D3 by 25-OHD3, some experiments indicated that 25-OHD3 might alter physiological processes when animals are challenged, for example, by a restricted mineral supply.
To investigate the influence of two dietary sources of vitamin D on the vitamin D status, bone metabolism, welfare and birth progress of gestating and lactating sows, forty-nine multiparous sows were randomly assigned to one of two diets: “CON” (n = 25; 50 μg vitamin D3/kg feed) and “HYD” (n = 24; 50 μg 25-hydroxycholecalciferol/kg feed). The basal diets were protein- and phosphorus-reduced. The trial started on day 3 ante insemination of the sows and ended with weaning of the piglets on day 28 postpartum. Dietary supplementation of 25-hydroxycholecalciferol resulted in improved maternal vitamin D status (p < 0.001), fewer gait changes (p < 0.01) and longer standing time after feeding (day 5 ante partum; p < 0.05) compared to vitamin D3. However, the bone markers CrossLaps and osteocalcin were not affected. Overall, the present results suggest that sows fed 25-hydroxycholecalciferol instead of vitamin D3 showed improved locomotion and stance strength. However, this outcome is probably not related to altered bone metabolism. The underlying mechanisms must be investigated in further studies.
Duckweeds can be potentially used in human and animal nutrition, biotechnology or wastewater treatment. To cultivate large quantities of a defined product quality, a standardized production process is needed. A small-scale, re-circulating indoor vertical farm (IVF) with artificial lighting and a nutrient control and dosing system was used for this purpose. The influence of different light intensities (50, 100 and 150 µmol m−2 s−1) and spectral distributions (red/blue ratios: 70/30, 50/50 and 30/70%) on relative growth rate (RGR), crude protein content (CPC), relative protein yield (RPY) and chlorophyll a of the duckweed species Lemna minor and Wolffiella hyalina were investigated. Increasing light intensity increased RGR (by 67% and 76%) and RPY (by 50% and 89%) and decreased chlorophyll a (by 27% and 32%) for L. minor and W. hyalina, respectively. The spectral distributions had no significant impact on any investigated parameter. Wolffiella hyalina achieved higher values in all investigated parameters compared to L. minor. This investigation proved the successful cultivation of duckweed in a small-scale, re-circulating IVF with artificial lighting.
Complete diets for laying hens are usually offered in meal form. This form initially promotes the laying hens’ natural feed intake behavior and allows them to satisfy their pecking behavior. At the same time, it can also cause difficulties, because it consists of different particles and is not a homogeneous unit. A homogeneous mixture is essential to ensure that each laying hen in the flock can meet its nutritional needs. If feed exhibits a wide particle size distribution, this can promote feed segregation during transport and selective feed intake behavior of laying hens. These two processes sometimes lead to significant differences between the composition of the feed produced and the composition of the feed that is finally ingested by the laying hens. Multi-stage sampling can be used to investigate progressing differences in feed composition. In this study, samples of different complete diets for laying hens (n = 76) were collected from ten organic farms in Germany to examine their particle size distributions (dry sieve analysis). Samples were taken at four different locations (V1 = loading, V2 = silo, V3 = at the beginning of the feed chain, V4 = at the end of the feed chain) in each farm. There was a tendency for V1 and V2 to be characterized by high proportions of particles between 1400 and 3150 µm (V1 = 61.2%, V2 = 43.5%). V3 and V4 consisted mainly of particles of size 500–800 µm and 200–400 µm, respectively. The lowest proportions across all variants were in the range above 3150 µm (V1 = 2.20%, V2 = 1.30%, V3 = 1.00%, V4 = 0.400%) and between 400 and 500 µm (V1 = 2.50%, V2 = 4.50%, V3 = 5.70%, V4 = 6.60%). The mean value comparison of the proportions of sieve mesh sizes from 200 to 1000 µm resulted in: V1 < V2 < V3 < V4; and of sieve mesh sizes between 1400 and 2000 µm in: V1 > V2 > V3 > V4. This observation can be explained by segregation of the feed during transport and a selective feeding behavior of the laying hens. However, trends were discontinuous and varied between the farms. Deviations from the guideline values were found in particular for particle sizes in the range of 1000 to 1400 µm.
Technological support options for the usage of Brazilian Açaí berries in the European Food Market
(2022)
The highly perishable fruit açaí grows on palm trees in northern Brazil and is colloquially known as a berry with high nutritional value. The seed of the drupe makes up around 85 percent of the fruits weight and only the pulp around the seed is used for human consumption. The manufacturing step after harvest includes the pulping and the preservation of the fruit. The preservation step is necessary, because the açaí pulp contains a high microbial load. There are several preservation processes including the use of chlorinated or ozonated water, alcoholic fermentation, pasteurization, freezing or dehydration. Those techniques are overall not very gentle and have the potential to leave residues in the final product, which can change its typical sensorial characteristics. Therefore, an experiment was conducted, to see if a relatively new gentle preservation method called PEF can reduce the microbial load in an açaí- smoothie.
For this purpose, a PEF-machine was built and verified based on the paper from HEINZ ET AL. [2003]. The self-built machine works efficiently, when there is a reduction of microorganisms like Escherichia coli in apple juice due to the induced Pulsed Electric Fields. If this is the case, the described experiment with açaí-smoothie can be carried out with the self-built PEF- machine. In this experiment the results of the validation of this PEF-machine were not comparable to those from the paper from HEINZ ET AL. [2003]. So, the self-built PEF-machine in Brazil did not work sufficiently. Hence, the experiment which should show that a reduction of microorganisms, such as Escherichia coli, in açaí-smoothie with PEF is possible, was performed in Germany. It was accrued out at ELEA with using the PEFPilotTM Dual. This experiment confirmed the assumption, that microorganisms can be reduced in açaí-smoothie with PEF. Escherichia coli was reduced by 2 logs, Saccharomyces cerevisiae by 3 logs and Lactobacillus plantarum by 6 logs. And a comparison between PEF and the known preservation methods for açaí showed that it can be a compatible alternative.
Moreover, the topic, how açaí fits into the European Food Market is answered within this paper. When offering açaí food products to the European population, ideas can be originated from the well-working Brazilian market. It can be helpful to mix açaí with known European fruits for a better acceptance by the people. Then açaí can help to meet the Europeans needs of the current time for fresh and healthy food, especially when preserved with PEF. Furthermore, it is important to work towards a sustainable supply chain system from the cultivation until the unloading at the destination in Europe. Sustainability is important for the integration in the European market, not only for environmental protection, but also in terms of social stability and marketing purposes. In addition, access requirements, further food-related regulations, and the seasonality of açaí present a major hurdle.
Building on this thesis, further papers shall be written, not only in the field of the preservation of the açaí pulp with PEF, but also in the direction of combined preservation methods for açaí, the sustainable usage of the açaí seeds, product innovations containing the Brazilian fruit or various market research.
Applications of pulsed electric fields for processing potatoes: Examples and equipment design
(2022)
In the last two decades, pulsed electric fields (PEF) have successfully been introduced into the food industry, as one of the most promising and "game changing" technologies. This review is devoted to the recent applications of pulsed electric fields used in processing potatoes. The potato processing market size was estimated to be ca. USD 24.83 billion (2018) and with an annual growth rate of 5.2%. The physicochemical characteristics of potatoes and the specificity of potato processing lines makes a pulsed electric field very versatile and flexible allowing one to achieve different technological aims by its implementation into technological lines. In this paper, a short analysis of the potato structure and its nutritional properties, applications of moderate electric fields, ohmic heating, and pulsed electric fields are presented. Moreover, the basic electroporation effects, metabolic responses, texture modification and different PEF assisted processes applied to the potato are discussed. Finally, some examples of commercial applications and a brief description of the available equipment for the PEF processing of potatoes are presented.
Повышение эффективности снятия покровной ткани с плодов томата импульсным электрическим полем
(2022)
Electrophysical technologies are a global trend of sustainable agriculture and food industry. Peeling is an energy-intensive procedure of fruit and vegetable processing. The research featured the effect of pulsed electric field (PEF) treatment on tomato peeling effectiveness. The assessment included such factors as specific effort, energy costs, and product losses in comparison with thermal and electrophysical methods. Tomatoes of Aurora variety underwent a PEF treatment at 1 kV/cm. The expended specific energy was 1, 5, and 10 kJ/kg. The tomatoes were visually evaluated with optical microscopy before and after processing. The peeling effectiveness and mass loss were measured with a texture analyzer and digital scales. The PEF treatment decreased the specific force of mechanical peel removal by 10% (P < 0.05). The mass loss decreased by 4% (P < 0.05) at 1 kJ/kg. The PEF method resulted in cell electroporation, which activated the internal mass transfer of moisture from the endocarp region between the mesocarp and the integumentary tissue. The hydrostatic pressure produced a layer of liquid, which facilitated the peeling. In comparison with thermal treatment (blanching), ohmic heating, and ultrasonic processing, the PEF technology had the lowest production losses and energy costs. The research proves the prospects of the PEF treatment in commercial tomato processing.
The impact of Pulsed Electric Field (PEF) processing pre-treatment on the texture and kinetics of in vitro starch digestibility of French fries made from two potato cultivars (Solanum tuberosum L.) containing dry matter content ranging from 19 to 22% was investigated. Whole and steam-peeled potato tubers were treated with a pilot scale PEF unit (electric field strength of 1.1 and 1.9 kV/cm with energy input <10 kJ/kg or ∼50 kJ/kg). This trial was carried out in a commercial French-fry plant using an industrial scale cutter, blancher, fryer and blast-freezer to prepare the frozen par-fried French fry samples. After subsequent final batch frying of the frozen fries, at 180 °C for 3 min to mimic the typical preparation practice at restaurant, retail and household, the outer crust of the fries produced from PEF-treated potatoes was significantly harder (9.4–16.3 N) than crust produced from untreated potatoes (6.9–8.5 N). High intensity (1.9 kV/cm with energy input ∼50 kJ/kg) PEF processing was found to cause defects (i.e. hollowness in the internal core) in the fries. A fractional conversion model was a good fit for the starch digestion kinetics of all French fry samples during the small intestinal phase (based on standardised INFOGEST static in vitro digestion assay). A lower % of total starch hydrolysis was predicted for French fries produced from high dry matter (>21%) tubers pretreated with PEF at electric field strength of 1.9 kV/cm. The findings generated in this study demonstrate PEF pretreatment may influence the texture of French fries and the extent of starch digestion that occurs.
Die Verschwendung von Lebensmitteln im Haushalt wird durch eine komplexe Reihe von routinemäßigen Verhaltensweisen bestimmt, und eine Unterbrechung dieser Routinen könnte zu einer Verringerung dieser enormen Menge an Lebensmittelabfällen führen. In der vorliegenden Studie wird eine solche Unterbrechung der Haushaltsroutinen untersucht: die Essensbox.
Das Potenzial von Essensboxen zur Verringerung verschiedener Arten von Lebensmittelabfällen in Haushalten wird zum ersten Mal in verschiedenen Ländern untersucht. Nachdem wir einen Rahmen für den Vergleich der Auswirkungen verschiedener Mahlzeitentypen auf die Lebensmittelverschwendung geschaffen haben, untersuchen wir anschließend die Auswirkungen einer abonnementbasierten Lebensmittelversorgung (d. h. Mahlzeitendosen) auf die gesamte Lebensmittelverschwendung sowie auf die verschiedenen Arten der Lebensmittelverschwendung: Zubereitung, Kochen und Tellerabfälle. Unser Datensatz enthält 8747 Beobachtungen von Mahlzeiten aus 955 Haushalten in sechs Ländern. Die Ergebnisse eines Bayes'schen Mehrebenen-Hurdle-Lognormal-Modells mit zufälligem Intercept zeigen, dass Essensboxen im Vergleich zu traditionell gekochten Mahlzeiten insgesamt zu einer Verringerung der gesamten Essensabfälle führen (38 % weniger). Mahlzeitendosen verringern vor allem das Auftreten und die Menge der verschwendeten Pfannen- und Topfgerichte (d. h. Kochabfälle) sowie die Menge der Mahlzeitenzubereitungsabfälle, führen jedoch im Vergleich zu herkömmlichen Mahlzeiten zu einem höheren Aufkommen von Zubereitungs- und Tellerabfällen. Dies zeigt, wie sich die Unterschiede zwischen den einzelnen Mahlzeiten auf die Lebensmittelabfälle in den Haushalten auswirken, ein Aspekt, der bisher in der Forschung wenig Beachtung gefunden hat. Während sich die meisten früheren Untersuchungen auf die gesamte Lebensmittelverschwendung in Haushalten konzentrierten, zeigt unsere Studie, dass die Unterscheidung zwischen verschiedenen Arten von Lebensmittelabfällen in Haushalten wichtige neue Erkenntnisse liefern kann.
Dieser Artikel befasst sich mit dem bundesweiten Niveau der Treibhausgas (THG)-Emissionen und des Ressourcenverbrauchs in der deutschen Gemeinschaftsverpflegung im Segment "Business" und deren Einsparpotenziale durch verschiedene Szenarien der ungestützten und gestützten Rezeptüberarbeitung. Unter "ungestützt" verstehen wir in dieser Arbeit die intuitive Optimierung von Rezepten durch die Mitarbeiter von Betrieben der Gemeinschaftsverpflegung. Die "unterstützten" Ansätze mussten dagegen bestimmte Zielvorgaben erfüllen, zum Beispiel der Deutschen Gesellschaft für Ernährung; engl. Deutschen Gesellschaft für Ernährung oder das nachhaltige Niveau. Konkret Konkret wird getestet, wie sich (A) eine ungestützte Rezeptüberarbeitung, (B) eine Rezeptüberarbeitung auf Basis von Ernährungsempfehlungen und (C) eine Rezeptüberarbeitung mit wissenschaftlicher Begleitung die Umweltauswirkungen eines Gerichtes beeinflussen. Als methodischer Rahmen diente eine Online-Befragung von Unternehmen der Gemeinschaftsverpflegung sowie eine Szenarioanalyse auf Menüebene und auf nationaler Ebene durchgeführt. Die Ergebnisse basieren zum einen auf empirischen Daten und zum anderen auf Hochrechnungen. Die Ergebnisse zeigen, dass die die bundesweite Umsetzung der Rezepturüberarbeitung nach wissenschaftlichen Vorgaben - wie konkrete Zielvorgaben für die THG Emissionen pro Portion - bis zu 44% des Ressourcenverbrauchs in der deutschen Betriebsgastronomie einsparen kann (das entspricht 3,4 Millionen Tonnen Ressourcen pro Jahr) und bis zu 40 % der THG-Emissionen (0,6 Millionen Tonnen THG-Emissionen pro Jahr) einsparen.
The study aimed to investigate inactivation of naturally occurring microorganisms and quality of red pepper paste treated by high pressure processing (HPP). Central composite rotatable design was employed to determine the impacts of pressure (100–600 MPa) and holding time (30–600 s). HPP at 527 MPa for 517 s reduced aerobic mesophilic bacteria count by 4.5 log CFU/g. Yeasts and molds counts were reduced to 1 log CFU/g at 600 MPa for 315 s. Total phenols, carotenoids and antioxidants activity ranged from 0.28 to 0.33 g GAE/100 g, 96.0–98.4 mg βc/100 g and 8.70–8.95 μmol TE/g, respectively. Increase (2.5–6.7%) in these variables was observed with increasing pressure and holding time. Total color difference (ΔE∗) values (0.2–2.8) were within the ranges of ‘imperceptible’ to ‘noticeable’. Experimental results were fitted satisfactorily into quadratic model with higher R2 values (0.8619–0.9863). Optimization process suggested treatment of red pepper paste at 536 MPa for 125 s for maximum desirability (0.622). Validation experiments confirmed comparable percentage of relative errors. Overall, this technique could be considered as an efficient treatment for the inactivation of microorganisms that naturally occur in red pepper paste with minimal changes in its characteristics.
Background
Spinach is a nitrogen (N) demanding crop with a weekly N uptake of up to 60 kg ha–1. Consequently, a high N supply is required, which can temporarily lead to high quantities of nitrate (NO3–) being at risk of leaching.
Aims
The objective of this study was to develop a N fertilization approach to reduce the risk of NO3– leaching in field-grown spinach production without adversely affecting crop yield and quality at an early and late harvest stage.
Methods
Ten fertilization trials were conducted to compare different base fertilization rates and splits of top dressings. For top dressings, granulated fertilizers or foliar sprays were used. In a further treatment, N supply was reduced by withholding the second top dressing of 50–70 kg ha−1.
Results
Nitrate concentration at risk of leaching was considerably reduced by decreasing the base fertilizer rate as well as by splitting the top dressing. However, at an early harvest stage, total aboveground dry mass was reduced by, on average, 6% by these measures across all seasons. In contrast, at a later harvest stage, spinach was less affected by the fertilizer schedule. Urea foliar sprays proved to be insufficient in promoting plant growth and caused leaf necrosis. A reduced N supply led to impaired plant growth and yellowish leaves in both spring and winter.
Conclusions
Base N fertilization of spinach is only required in spring, but not in other seasons. Despite slight yield reduction, the top dressing should be split to reduce the risk of NO3− leaching after an early harvest.
Intensive care units (ICU) are often overflooded with alarms from monitoring devices which constitutes a hazard to both staff and patients. To date, the suggested solutions to excessive monitoring alarms have remained on a research level. We aimed to identify patient characteristics that affect the ICU alarm rate with the goal of proposing a straightforward solution that can easily be implemented in ICUs. Alarm logs from eight adult ICUs of a tertiary care university-hospital in Berlin, Germany were retrospectively collected between September 2019 and March 2021. Adult patients admitted to the ICU with at least 24 h of continuous alarm logs were included in the study. The sum of alarms per patient per day was calculated. The median was 119. A total of 26,890 observations from 3205 patients were included. 23 variables were extracted from patients' electronic health records (EHR) and a multivariable logistic regression was performed to evaluate the association of patient characteristics and alarm rates. Invasive blood pressure monitoring (adjusted odds ratio (aOR) 4.68, 95%CI 4.15–5.29, p < 0.001), invasive mechanical ventilation (aOR 1.24, 95%CI 1.16–1.32, p < 0.001), heart failure (aOR 1.26, 95%CI 1.19–1.35, p < 0.001), chronic renal failure (aOR 1.18, 95%CI 1.10–1.27, p < 0.001), hypertension (aOR 1.19, 95%CI 1.13–1.26, p < 0.001), high RASS (aOR 1.22, 95%CI 1.18–1.25, p < 0.001) and scheduled surgical admission (aOR 1.22, 95%CI 1.13–1.32, p < 0.001) were significantly associated with a high alarm rate. Our study suggests that patient-specific alarm management should be integrated in the clinical routine of ICUs. To reduce the overall alarm load, particular attention regarding alarm management should be paid to patients with invasive blood pressure monitoring, invasive mechanical ventilation, heart failure, chronic renal failure, hypertension, high RASS or scheduled surgical admission since they are more likely to have a high contribution to noise pollution, alarm fatigue and hence compromised patient safety in ICUs.
Background
To detect changes in biological processes, samples are often studied at several time points. We examined expression data measured at different developmental stages, or more broadly, historical data. Hence, the main assumption of our proposed methodology was the independence between the examined samples over time. In addition, however, the examinations were clustered at each time point by measuring littermates from relatively few mother mice at each developmental stage. As each examination was lethal, we had an independent data structure over the entire history, but a dependent data structure at a particular time point. Over the course of these historical data, we wanted to identify abrupt changes in the parameter of interest - change points.
Results
In this study, we demonstrated the application of generalized hypothesis testing using a linear mixed effects model as a possible method to detect change points. The coefficients from the linear mixed model were used in multiple contrast tests and the effect estimates were visualized with their respective simultaneous confidence intervals. The latter were used to determine the change point(s). In small simulation studies, we modelled different courses with abrupt changes and compared the influence of different contrast matrices. We found two contrasts, both capable of answering different research questions in change point detection: The Sequen contrast to detect individual change points and the McDermott contrast to find change points due to overall progression. We provide the R code for direct use with provided examples. The applicability of those tests for real experimental data was shown with in-vivo data from a preclinical study.
Conclusion
Simultaneous confidence intervals estimated by multiple contrast tests using the model fit from a linear mixed model were capable to determine change points in clustered expression data. The confidence intervals directly delivered interpretable effect estimates representing the strength of the potential change point. Hence, scientists can define biologically relevant threshold of effect strength depending on their research question. We found two rarely used contrasts best fitted for detection of a possible change point: the Sequen and McDermott contrasts.
Background
A peripheral venous catheter (PVC) is the most widely used device for obtaining vascular access, allowing the administration of fluids and medication. Up to 25% of adult patients, and 50% of pediatric patients experience a first-attempt cannulation failure. In addition to patient and clinician characteristics, device features might affect the handling and success rates. The objective of the study was to compare the first-attempt cannulation success rate between PVCs with wings and a port access (Vasofix® Safety, B. Braun, abbreviated hereon in as VS) with those without (Introcan® Safety, B. Braun, abbreviated hereon in as IS) in an anesthesiological cohort.
Methods
An open label, multi-center, randomized trial was performed. First-attempt cannulation success rates were examined, along with relevant patient, clinician, and device characteristics with univariate and multivariate analyses. Information on handling and adherence to use instructions was gathered, and available catheters were assessed for damage.
Results
Two thousand three hundred four patients were included in the intention to treat analysis. First-attempt success rate was significantly higher with winged and ported catheters (VS) than with the non-winged, non-ported design (IS) (87.5% with VS vs. 78.2% with IS; PChi < .001). Operators rated the handling of VS as superior (rating of “good” or “very good: 86.1% VS vs. 20.8% IS, PChi < .001). Reinsertion of the needle into the catheter after partial withdrawal—prior or during the catheterization attempt—was associated with an increased risk of cannulation failure (7.909, CI 5.989–10.443, P < .001 and 23.023, CI 10.372–51.105, P < .001, respectively) and a twofold risk of catheter damage (OR 1.999, CI 1.347–2.967, P = .001).
Conclusions
First-attempt cannulation success of peripheral, ported, winged catheters was higher compared to non-ported, non-winged devices. The handling of the winged and ported design was better rated by the clinicians. Needle reinsertions are related to an increase in rates of catheter damage and cannulation failure.
During gestation, the most drastic change in oxygen supply occurs with the onset of ventilation after birth. As the too early exposure of premature infants to high arterial oxygen pressure leads to characteristic diseases, we studied the adaptation of the oxygen sensing system and its targets, the hypoxia-inducible factor- (HIF-) regulated genes (HRGs) in the developing lung. We draw a detailed picture of the oxygen sensing system by integrating information from qPCR, immunoblotting, in situ hybridization, and single-cell RNA sequencing data in ex vivo and in vivo models. HIF1α protein was completely destabilized with the onset of pulmonary ventilation, but did not coincide with expression changes in bona fide HRGs. We observed a modified composition of the HIF-PHD system from intrauterine to neonatal phases: Phd3 was significantly decreased, while Hif2a showed a strong increase and the Hif3a isoform Ipas exclusively peaked at P0. Colocalization studies point to the Hif1a-Phd1 axis as the main regulator of the HIF-PHD system in mouse lung development, complemented by the Hif3a-Phd3 axis during gestation. Hif3a isoform expression showed a stepwise adaptation during the periods of saccular and alveolar differentiation. With a strong hypoxic stimulus, lung ex vivo organ cultures displayed a functioning HIF system at every developmental stage. Approaches with systemic hypoxia or roxadustat treatment revealed only a limited in vivo response of HRGs. Understanding the interplay of the oxygen sensing system components during the transition from saccular to alveolar phases of lung development might help to counteract prematurity-associated diseases like bronchopulmonary dysplasia.
Objectives: To measure and assess the economic impact of adherence to a single quality indicator (QI) regarding weaning from invasive ventilation.
Design: Retrospective observational single-centre study, based on electronic medical and administrative records.
Setting: Intensive care unit (ICU) of a German university hospital, reference centre for acute respiratory distress syndrome.
Participants: Records of 3063 consecutive mechanically ventilated patients admitted to the ICU between 2012 and 2017 were extracted, of whom 583 were eligible adults for further analysis. Patients’ weaning protocols were evaluated for daily adherence to quality standards until ICU discharge. Patients with <65% compliance were assigned to the low adherence group (LAG), patients with ≥65% to the high adherence group (HAG).
Primary and secondary outcome measures: Economic healthcare costs, clinical outcomes and patients’ characteristics.
Results: The LAG consisted of 378 patients with a median negative economic results of −€3969, HAG of 205 (−€1030), respectively (p<0.001). Median duration of ventilation was 476 (248; 769) hours in the LAG and 389 (247; 608) hours in the HAG (p<0.001). Length of stay (LOS) in the LAG on ICU was 21 (12; 35) days and 16 (11; 25) days in the HAG (p<0.001). LOS in the hospital was 36 (22; 61) days in the LAG, and within the HAG, respectively, 26 (18; 48) days (p=0.001).
Conclusions: High adherence to this single QI is associated with better clinical outcome and improved economic returns. Therefore, the results support the adherence to QI. However, the examined QI does not influence economic outcome as the decisive factor.
The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to d4 are allergens present in a variety of different isoforms in apples. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. A total of 195, 17, 14, and 18 peptides were found to be related to Mal d1, d2, d3, and d4 proteins, respectively of which 25 different Mal d proteins could be unambiguously identified. The allergenic potential of the Mal d isoforms was characterized by comparing the isoform abundance with the allergenic score of genotypes from oral challenge tests. The detected Mal d peptides presumably have different IgE binding properties and could be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars.
Animal husbandry methods also play an important role in public discussion, as animal welfare is often valued in society by visual perceptions. In this context, there is often an idealized idea of livestock husbandry and nutrition, which is staged by ideal-typical images. In the minds of many citizens, nature-loving images trigger a positive imagination that results from the longings of urban living conditions. Media and stakeholder analyses indicate that the use of straw in livestock husbandry and nutrition also has a positive impact on the welfare of livestock. According to this, straw is preferred by the public for more animal welfare. But what is not considered is the fact that the straw must be of impeccable hygienic quality
Die vorliegende Masterarbeit befasst sich mit dem Thema der Darstellung von Vegetation im Kontext des Building Information Modeling (BIM) und hierbei insbesondere mit der Entwicklung eines Datenmodells für ausgewählte Baumarten.
Die Anwendung der BIM-Methode wird in der Landschaftsarchitektur zunehmend gefordert. Zurzeit sind die Integration und die Arbeit mit der BIM-Methode noch mit großen Herausforderungen verbunden. Für die Landschaftsarchitektur fehlen definierte Objekte im internationalen, herstellerneutralen Austauschformat Industry Foundation Classes (IFC). Es fehlen neben den Objekten des Freiraums die Workflows zur Modellierung der Objekte, die beschreibenden Informationen und die Zuordnung zu den Detaillierungsgraden.
Das Ziel der vorliegenden Masterarbeit besteht darin, eine Methode zum Generieren von parametrischen Baummodellen zu entwickeln. Es werden individuelle Baummodelle in der CAD/BIM-Software Autodesk Revit modelliert. Die Baummodelle sollen den Lebenszyklus einer Pflanze abbilden. Das in dieser Arbeit erstellte Baummodell ermöglicht die Abbildung einer Vielzahl von Kronen- und Wurzelformen. Die generierten Baummodelle beinhalten Wachstumsfunktionen mit spezifischen Parametern für die Gesamthöhe, die lichte Stammhöhe, den größten Kronendurchmesser, die Höhe des größten Kronendurchmessers sowie Länge und Tiefe der Wurzel. Zusätzlich zur Geometrie des Modells werden relevante Informationen als konsistenter Parametersatz definiert. Die Informationen können den BIM-Anwendungsfällen zugeordnet werden. Die Parametersätze für das Erscheinungsbild der Modelle und die Informationen zu den Eigenschaften werden über Dynamo gesteuert. Das ermöglicht den direkten Austausch der Modelle und der zugehörigen Informationen.
Ein erläuterndes Video (HAVERLAND 2022) zur Vorgehensweise und den Ergebnissen findet sich unter https://youtu.be/Azx3mPMu3Ng
Die vorliegende Arbeit befasst sich mit dem BIM-basierten (Building Information Modeling) Bauantrag und der Frage, welche Anforderungen hierfür bei BIM-Modellen der Außenanlagenplanung zu berücksichtigen sind.
Das Baugenehmigungsverfahren, wie man es heute kennt, ist zeit-, kosten- und personalintensiv sowie von langen Laufzeiten geprägt. Im Hinblick auf aktuelle Herausforderungen für die Planungs- und Baubranche wie Klimaanpassung, Verkehrswende und Wohnungsnot bei gleichzeitig immer komplexer werdenden bauordnungs- und bauplanungsrechtlichen Anforderungen ist eine Modernisierung des Bauantragsverfahrens unabdingbar. Mit dem Standardisierungsprojekt ,Austauschstandards im Bau- und Planungsbereich‘ des IT-Planungsrates wird daher seit einigen Jahren die Digitalisierung des Baugenehmigungsverfahrens vorangetrieben. So soll der Austausch von Daten und Informationen zwischen allen Beteiligten (planende Stelle, Genehmigungsstelle Antragsteller*innen usw.) zukünftig digital und unter bundesweit verbindlichen, herstellerneutralen, offenen Datenstandards erfolgen. Gleichzeitig ermöglicht die Einführung des digitalen Baugenehmigungsverfahrens auch die Einbindung von BIM-Modellen. Wie diese im digitalen Bauantragsverfahren genutzt werden können, welche Anforderungen diese erfüllen müssen und welche Chancen und Mehrwerte dadurch entstehen (z. B. durch die teilautomatisierte Regelprüfung von bauordnungs- und bauplanungsrechtlichen Anforderungen) wurde bereits im Forschungsprojekt ‚BIM-basierter Bauantrag‘ im Rahmen der Forschungsinitiative ‚Zukunft Bau‘ untersucht. Hierbei wurden allerdings insbesondere Gebäudemodelle betrachtet.
Zunehmend wird die Anwendung der BIM-Methode jedoch auch von der Landschaftsarchitektur gefordert. Aufgrund bisher nicht definierter Objekte im internationalen, herstellerneutralen Austauschstandard von BIM-Modellen IFC (Industry Foundation Classes) für den Bereich Landschaft, ist die Teilhabe an BIM-Prozessen zurzeit noch eine große Herausforderung für die Landschaftsarchitektur. So fehlen neben Objekten des Freiraums Standards sowohl hinsichtlich der Modellierung als auch für die zu beschreibenden Attribute in den erforderlichen Detaillierungsgraden.
Ziel dieser Arbeit ist es daher, Anforderungen an Außenanlagenmodelle für den BIM-basierten Bauantrag zu untersuchen und in eine Modellierungsrichtlinie umzusetzen. Ein weiteres Ziel ist es, mögliche Chancen der teilautomatisierten Regelprüfung von Bauvorschriften für Außenanlagen aufzuzeigen. Hierfür erfolgt zunächst eine Dokumentenanalyse von verschiedenen Bauvorlagenverordnungen. Des Weiteren werden auch die Vorgaben für den Detaillierungsgrad LOD200 (Level of Detail) untersucht. Die Umsetzung der Modellierungsrichtlinie erfolgt exemplarisch am Beispiel der Außenanlagenplanung des Bauvorhabens ‚Elbtower‘ in Hamburg. An diesem Beispiel werden die für den Bauantrag relevanten Objekte des Freiraums und deren geometrischen und semantischen Anforderungen identifiziert. Für Objekte, die bisher nicht im IFC-Standard klassifiziert sind, werden zudem Klassifizierungsvorschläge gemacht, um eine mögliche Aufnahme in die IFC zu forcieren. Die Umsetzbarkeit der Modellierungsrichtlinie wird beispielhaft mit der BIM-Software ‚Autodesk Revit´ verifiziert. Abschließend werden die Möglichkeiten der teilautomatisierten Regelprüfung von bauordnungs- und bauplanungsrechtlichen Anforderungen an Außenanlagenmodelle anhand exemplarisch ausgewählter Prüffälle untersucht.
Animal husbandry methods also play an important role in public discussion, as animal welfare is often valued in society by visual perceptions. In this context, there is often an idealized idea of livestock husbandry and nutrition, which is staged by ideal-typical images. In the minds of many citizens, nature-loving images trigger a positive imagination that results from the longings of urban living conditions. Media and stakeholder analyses indicate that the use of straw in livestock husbandry and nutrition also has a positive impact on the welfare of livestock. According to this, straw is preferred by the public for more animal welfare.
But what is not considered is the fact that the straw must be of impeccable hygienic quality. Fungal infestation and the formation of mycotoxins in straw can cause diseases in livestock with consequences for animal welfare.
The first evaluation of a perfect straw quality also takes place in science through sensory tests, i.e. through smell, grip, colour and impurities. Only in the case of abnormalities in the sensory tests are further examinations indicated, such as microbiological examination procedures.
The hygienic properties of straw were examined on the basis of these assessment criteria. In addition to the microbiological-hygienic tests, the sensors of the straw were also tested.
The results show that there are no abnormalities in the sensory examination of the hygiene status. This was to observe an impeccable hygiene status.
However, the microbiological-hygienic investigations showed that the straw had microbiological as well as mycotoxin loads above the orientation values. This can have negative health effects, such as diseases for farm animals.
The scientific results led to the conclusion: The public discussion about animal welfare, which is often conducted primarily on the basis of visual impressions, could gain in scientific resilience if it includes objective results such as microbiological analyses in addition to images in order to evaluate animal welfare in livestock farming
Chitin is an abundant waste product from shrimp and mushroom industries and as such, an appropriate secondary feedstock for biotechnological processes. However, chitin is a crystalline substrate embedded in complex biological matrices, and, therefore, difficult to utilize, requiring an equally complex chitinolytic machinery. Following a bottom-up approach, we here describe the step-wise development of a mutualistic, non-competitive consortium in which a lysine-auxotrophic Escherichia coli substrate converter cleaves the chitin monomer N-acetylglucosamine (GlcNAc) into glucosamine (GlcN) and acetate, but uses only acetate while leaving GlcN for growth of the lysine-secreting Corynebacterium glutamicum producer strain. We first engineered the substrate converter strain for growth on acetate but not GlcN, and the producer strain for growth on GlcN but not acetate. Growth of the two strains in co-culture in the presence of a mixture of GlcN and acetate was stabilized through lysine cross-feeding. Addition of recombinant chitinase to cleave chitin into GlcNAc2, chitin deacetylase to convert GlcNAc2 into GlcN2 and acetate, and glucosaminidase to cleave GlcN2 into GlcN supported growth of the two strains in co-culture in the presence of colloidal chitin as sole carbon source. Substrate converter strains secreting a chitinase or a β-1,4-glucosaminidase degraded chitin to GlcNAc2 or GlcN2 to GlcN, respectively, but required glucose for growth. In contrast, by cleaving GlcNAc into GlcN and acetate, a chitin deacetylase-expressing substrate converter enabled growth of the producer strain in co-culture with GlcNAc as sole carbon source, providing proof-of-principle for a fully integrated co-culture for the biotechnological utilization of chitin.
Notable parts of the population in Europe suffer from allergies towards apples. To address this health problem, the analysis of the interactions of relevant allergens with other substances such as phenolic compounds is of particular importance. The aim of this study was to evaluate the correlations between the total phenolic content (TPC), polyphenol oxidase (PPO) activity, antioxidant activity (AOA), and the phenolic compound profile and the content of the allergenic protein Mal d 1 in six apple cultivars. It was found that the PPO activity and the content of individual phenolic compounds had an influence on the Mal d 1 content. With regard to the important constituents, flavan-3-ols and phenolic acids, it was found that apples with a higher content of chlorogenic acid and a low content of procyanidin trimers and/or epicatechin had a lower allergenic potential. This is probably based on the reaction of phenolic compounds (when oxidized by the endogenous PPO) with proteins, thus being able to change the conformation of the (allergenic) proteins, which further corresponds to a loss of antibody recognition. When apples were additionally biofortified with selenium, the composition of the apples, with regard to TPC, phenolic profile, AOA, and PPO, was significantly affected. Consequently, this innovative agronomic practice seems to be promising for reducing the allergenic potential of apples.
Selenium and iodine are essential micronutrients for humans. They are often deficient in food supply due to low phytoavailable concentrations in soil. Agronomic biofortification of food
crops is one approach to overcome micronutrient malnutrition. This study focused on a pre-launch exploration of German consumers’ willingness to purchase selenium- and/or iodine-biofortified apples. For this purpose, an online survey was carried out. In this context, consumers were asked to choose their most preferred apple product from a set card of product alternatives in a discrete choice experiment (DCE). The multinomial logit model results demonstrated that German consumers’ have a particular preference for iodine-biofortified apples. Furthermore, apple choice was mainly influenced by price, health claims, and plastic-free packaging material. Viewed individually, selenium did not exert an effect on product choice whereas positive interactions between both micronutrients exist.
Die Direktvermarktung von landwirtschaftlichen Erzeugnissen spielt in Niedersachsen eine bedeutende Rolle zur Einkommensaufwertung der landwirtschaftlichen Betriebe. Das Internet ist dabei mittlerweile ein entscheidendes Medium zur Kundenansprache. Diese Studie hat daher die Zielstellung, die Bildkommunikation der landwirtschaftlichen Direktvermarkter in Niedersachsen im Rahmen der klassischen Homepage zu untersuchen. Entsprechendes Bildmaterial der Erstansicht der Homepages von Direktvermarktungsbetrieben wurde dafür einer qualitativen und quantitativen Analyse unterzogen. 258 Homepages von niedersächsischen Direktvermarktern wurden in die Auswertung einbezogen. Die Auswertung zeigt: Mehr als jeder zweite niedersächsische Direktvermarkter stellt Gebäude auf der Erstansicht seiner Webseite dar. Es handelt sich hierbei überwiegend um historisch aussehende Gebäude. Weiterhin konnte ermittelt werden, dass neben Gebäuden auch Personen, Tiere, Obst, Gemüse, Pflanzen, Produkte sowie Landschaften auf den Erstansichten der Webseiten dargestellt werden.
Mountainbiking ist eine stark wachsende Natursportart mit einer hohen Teilnehmerzahl an Downhill- Mountainbikern im nördlichen Harz. Aufgrund der Wegebelastung entstehen soziale Konflikte und ökologische Auswirkungen, weshalb attraktive Strecken entwickelt und umweltschonende Lenkungsmaßnahmen durchgeführt werden müssen. Die Umfrage, die im Zuge dieser Arbeit durchgeführt wurde, ergab, dass die Anforderungen der Mountainbiker naturnahe, schmale und technisch anspruchsvolle Strecken sind. Die Auswirkungen des Mountainbikings betreffen die Tierwelt und sensible Gebiete abseits vorhandener Wege. Für die erfolgreiche Lenkung der Mountainbiker ist Kommunikation zwischen allen Beteiligten in der Landschaftsplanung erforderlich. Es ist auf eine umweltschonende Bauweise, gezielte Streckenführung, klare Beschilderungen, technische Herausforderungen und Wegequalitäten zu achten. Der Ausbau vorhandener Wegeinfrastruktur und aktive Lenkungsmaßnahmen im Harz können zu einer konfliktfreien und umweltfreundlichen Situation des Mountainbikings führen. Zusätzliche Forschungsarbeiten mit konkreten Schwerpunkten einzelner Lenkungskriterien oder ökologischen Auswirkungen können das Management von Mountainbike- Strecken erleichtern.
1. Flower strips are a fundamental part of agri-environment schemes (AESs) introduced by the European Union to counteract the loss of biodiversity and related ecosystem services in agricultural landscapes. Although vegetation composition of the strips is essential for most fauna groups, comprehensive studies analysing vegetation development and influencing factors are rare.
2. From 2017 to 2019, we investigated the vegetation composition of 40 perennial wildflower strips (WFSs) implemented in 2015 or 2016, and 20 cereal fields without WFS across Saxony-Anhalt, Germany. We analysed environmental factors on plot (cover of grasses, shading, soil fertility) and four landscape-scale levels (habitat diversity, proportion of WFS and open habitats). The provision of nectar and pollen resources was estimated by the newly developed Pollinator Feeding Index (PFI). All strips had been implemented by farmers as AES with species- rich seed mixtures comprising 30 native forbs.
3. In all study years, forb species richness, cover and related nectar and pollen supply were much higher on WFSs than on controls, confirming the effectiveness of this AES. Although sown native forbs contributed the most to the high PFI values, spontaneously established forbs expanded the total range of species considerably, especially in winter and spring. While sown forb communities remained similar over time, spontaneous forbs showed a higher species turnover. Altogether, shading and grass cover had the greatest negative effect on the performance of the sown forbs. Landscape variables had only minor effects and were inconsistent in their importance across scale levels and years.
4. Synthesis and applications. Successfully established perennial wildflower strips (WFSs) sown with species-rich native seed mixtures provided a forb-rich and diverse vegetation throughout the AES funding period of 5 years. By supplying feeding resources for pollinators under various landscape situations, WFSs have significant potential to promote farmland biodiversity and related ecosyste services. We recommend the mandatory use of species-rich wildflower mixtures for perennial flower strips and to avoid their creation in heavily shaded field edges. Advisory services for farmers are necessary to prevent failures in WFS implementation and management and to improve their ecological effectiveness.
High levels of meat consumption are increasingly being criticised for ethical, environmental, and social reasons. Plant-based meat substitutes have been with reservations identified as healthy sources of protein in comparison to meat. This alternative offers several social, environmental, and probably health benefits, and it may play a role in reducing meat consumption. However, there has been a lack of research on how specific meat substitute attributes can influence consumers to replace or partially replace meat in their diets. Research has demonstrated that, in many countries, consumers are highly attached to meat. They consider it to be an essential and integral element of their daily diet. For the consumers that are not interested in vegan or vegetarian alternatives to meat, so-called meathybrids could be a low-threshold option for a more sustainable food consumption behaviour. In meathybrids, only a fraction of the meat product (e.g., 20% to 50%) is replaced with plant-based proteins. In this paper, the results of an online survey with 500 German consumers are presented with a focus on preferences and attitudes relating to meathyrids. The results show that more than fifty percent of consumers substitute meat at least occasionally. Thus, approximately half of the respondents reveal an eligible consumption behaviour with respect to sustainability and healthiness to a certain degree. Regarding the determinants of choosing either meathybrid or meat, it becomes evident that the highest effect is exerted by the health perception. The healthier meathybrids are perceived, the higher is the choice probability. Thus, this egoistic motive seems to outperform altruistic motives, like animal welfare or environmental concerns, when it comes to choice for this new product category.
Duckweed is a promising resource for future feed and food production as well as wastewater treatment. However, diseases and pests can critically limit the performance of the production systems. Patches of discolored and bleached duckweed (Lemna minor L.) appeared in hydroponic systems and spread rapidly through the crop. Pythium myriotylum was confirmed as the causing pathogen by microbiological and molecular biological analysis. This is the first report of P. myriotylum on duckweed in Germany. The result and possible countermeasures are discussed.
In order to produce protein-rich duckweed for human and animal consumption, a stable cultivation process, including an optimal nutrient supply for each species, must be implemented. Modified nutrient media, based on the N-medium for duckweed cultivation, were tested on the relative growth rate (RGR) and crude protein content (CPC) of Lemna minor and Wolffiella hyalina, as well as the decrease of nitrate-N and ammonium-N in the media. Five different nitrate-N to ammonium-N molar ratios were diluted to 10% and 50% of the original N-medium concentration. The media mainly consisted of agricultural fertilizers. A ratio of 75% nitrate-N and 25% ammonium-N, with a dilution of 50%, yielded the best results for both species. Based on the dry weight (DW), L. minor achieved a RGR of 0.23 ± 0.009 d−1 and a CPC of 37.8 ± 0.42%, while W. hyalina’s maximum RGR was 0.22 ± 0.017 d−1, with a CPC of 43.9 ± 0.34%. The relative protein yield per week and m2 was highest at this ratio and dilution, as well as the ammonium-N decrease in the corresponding medium. These results could be implemented in duckweed research and applications if a high protein content or protein yield is the aim.
Die Analyse der kommunalen Planungspraxis zur Auswahl und Bewertung von Siedlungsflächen in der Flächennutzungsplanung zeigt auf, welchen Einfluss Forschungsergebnisse auf die aktuelle Planungspraxis haben. Aufbauend auf einer Analyse des Forschungsstandes und der rechtlichen Rahmenbedingungen wird die Bewertung von Siedlungsflächen in 29 verschiedenen Flächennutzungsplänen untersucht. Als Ergebnis wurde ermittelt, dass in der Planungspraxis die Anzahl der Bewertungskriterien und die Komplexität der Bewertung in den letzten Jahren stark zugenommen hat. Außerdem kann gezeigt werden, dass und wie stark sich die Planungsmethodik und die verwendeten Kriterien zur Auswahl und Bewertung von Siedlungsflächen zwischen den einzelnen Gemeinden unterscheiden. Trotz umfangreicher anwendungsorientierter Forschung seit den 1990er Jahren zur Bewertung und Auswahl von Siedlungsflächen lassen sich jedoch nur indirekte Einflüsse der Forschungsergebnisse auf die Planungspraxis identifizieren. Teilweise wurde allerdings festgestellt, dass auch von den Gemeinden selbst neue Kriterien und Vorgehensweisen entwickelt werden.
Die Landwirtschaft ist ein Wirtschaftszweig mit massiven Auswirkungen auf die biologische und agrobiodiverse Vielfalt. Nachhaltige Ernährung ist ein entscheidender politischer Hebel und eine realistische Chance, die Umweltauswirkungen des Agrar- und Ernährungssektors zu verringern und gleichzeitig die menschliche Gesundheit zu verbessern. Auswärts essen ist für viele Verbraucher eine immer häufiger anzutreffende Gewohnheit, und indem sie nachhaltige Gerichte anbieten, können Catering-Unternehmen eine zentrale Rolle spielen. Dazu müssen sie die Nachhaltigkeit ihres Lebensmittelangebots verstehen und richtig einschätzen können, aber es gibt noch keine gut etablierten Bewertungsinstrumente. Im Rahmen des NAHGAST-Projekts, zu dem diese Studie gehörte, wurde ein Instrument zur Bewertung der Nachhaltigkeit für Catering-Unternehmen entwickelt und getestet, das auf konkreten, pro Mahlzeit definierten Zielen basiert. Diese Studie befasst sich mit dem Mangel an Methoden zur Bewertung der Auswirkungen von Lebensmitteln auf die biologische Vielfalt, wobei der Schwerpunkt auf der Agrobiodiversität liegt. Die Arbeit veranschaulicht eine kontextspezifische Anwendung eines erweiterten DPSIR-Modells zur Strukturierung von Informationen und Auswahl von Indikatoren und schlägt eine transdisziplinäre Nutzung bestehender Messgrößen vor. Weitere Forschungsarbeiten sind erforderlich, um wissenschaftlich fundierte Zielwerte oder Nachhaltigkeitsspannen für jeden Indikator pro Mahlzeit zu definieren, damit diese berechnet werden können.
Die Stärken und Grenzen der Studie werden diskutiert.
Quantitative Umweltbewertungen sind von entscheidender Bedeutung, wenn es darum geht, effektiv auf nachhaltige Produktions- und Verbrauchsmuster hinzuarbeiten. In den letzten Jahrzehnten haben sich Lebenszyklusanalysen (LCA) als praktikables Mittel zur Messung der Umweltauswirkungen von Produkten entlang der Lieferkette etabliert. In Bezug auf Nutzer- und Konsummuster wurden jedoch methodische Schwächen festgestellt, und es wurden mehrere Versuche unternommen, Ökobilanzen entsprechend zu verbessern, beispielsweise durch Einbeziehung von Effekten höherer Ordnung und verhaltenswissenschaftlicher Unterstützung. In einer Diskussion solcher Ansätze zeigen wir, dass den Konzepten des Verbrauchs keine explizite Aufmerksamkeit geschenkt wurde, was häufig zu produktzentrierten Bewertungen führt. Wir führen Theorien sozialer Praktiken ein, um Konsummuster für LCA zugänglich zu machen. Soziale Praktiken sind routinierte Handlungen, die aus miteinander verbundenen Elementen (Materialien, Kompetenzen und Bedeutungen) bestehen, die sie als eine Einheit denkbar machen (z. B. Kochen). Da die meisten sozialen Praktiken eine Art von Verbrauch (Material, Energie, Luft) beinhalten, konnten wir einen Rahmen entwickeln, der soziale Praktiken mit der Ökobilanz verbindet. Der vorgeschlagene Rahmen bietet eine neue Perspektive für quantitative Umweltbewertungen, indem er den Schwerpunkt von Produkten oder Nutzern auf soziale Praktiken verlagert. Dementsprechend sehen wir die Chance darin, die reduktionistische Sichtweise zu überwinden, dass Menschen nur Nutzer von Produkten sind, und sie stattdessen als Praktiker in sozialen Praktiken zu sehen. Dieser Wandel könnte neue Methoden der interdisziplinären Konsumforschung ermöglichen, die beabsichtigte Sozialwissenschaften und wirkungsorientierte Bewertungen einbeziehen. Allerdings bedarf der Rahmen einer weiteren Überarbeitung und vor allem einer empirischen Validierung.
Der Konsumbereich „Ernährung“ verantwortet in Deutschland rund 15 % der Treibhausgasemissionen (THG). Aufgrund der hohen Nachfrage hat die Außer-Haus-Verpflegung eine starke Hebelwirkung, um eine klimaeffiziente Ernährung voranzutreiben – so auch Schulküchen. Aktuell sind der Verpflegung jedes Schulkindes, welches ganzjährig an der Mittagsverpflegung teilnimmt, pro Jahr etwa 264 kg THG-Emissionen zuzuschreiben.
Im Projekt KEEKS „Klima- und energieeffiziente Küche in Schulen” wurden deshalb in 22 Schulküchen von Ganztagsschulen mit täglich insgesamt 5 000 Mittagessen der Status Quo ermittelt. Dies geschah mittels Energiemessungen, Analyse von Ausstattung, Technik und Prozessen der Küchen sowie leitfadengestützten Interviews mit den Küchenleitungen. Treibhausgasemissionen von Menüs und Küchenprozessen wurden bilanziert, Einsparpotenziale identifiziert sowie Handlungsempfehlungen entwickelt und erprobt. Die effektivsten Maßnahmen – die Reduktion und Substitution von Fleisch und Fleischprodukten sowie der Aufbau eines effizienten Abfallmanagements – sparen rund 10 % des Treibhausgasausstoßes einer Schulküche ein. Die erarbeiteten Handlungsempfehlungen können Küchenpersonal dabei unterstützen, eine klimafreundliche, kindgerechte, gesunde und bezahlbare Schulküche zu gestalten.
Red pepper (Capsicum annuum L.) is one of the major spices consumed globally, recognized for its aroma and nutrient properties, and it has a major economic value for high producing countries. However, characterization of its techno-functional properties and in-depth understanding of oxidative stability is needed to produce food of high quality and stability. Thus, this work focused on the chemical, functional, thermal, oxidative stability and rheological properties of red pepper powder and paste. Experiment was designed in a Completely Randomized Design (CRD) fashion. The red pepper powder contained 14.50 g/100 g, 44.00 g/100 g and 7.57 g/100 g of crude fat, crude fiber and ash, respectively. The concentration of total phenols, carotenoids and antioxidants activity of the powder were 1.04 g GAE/100 g, 374 mg βc/100 g and 38.61 μmol TE/g, respectively. Functional properties showed lower bulk density (395.1 kg/m3) and higher tapped density (583.4 kg/m3) indicating the higher compressibility of the powder. In contrast, Hausner ratio (1.48), Carr’s index (32%) and angle of repose (45°) indicated poor flowability of the powder. Particle size distribution also indicated that the volume weighted mean values D[4,3] of the powder and paste were 262.20 and 201.46, respectively. Emulsifying capacity of the powder was 47.5%. Oil and water absorption capacities varied from 1.41 to 1.73 and 0.86 to 2.29 g/g of initial weight, respectively. Higher glass transition temperature was observed for the powder (62.54°C) than the paste (45.64°C). The induction period indicated that red pepper was more stable against oxidation in powder (5.2 h) than in the paste form (3.2 h). Rheological analysis revealed that the paste exhibited shear-thinning behavior. Overall, understanding of the properties of red pepper could contribute to enhance quality.
The increased consumption of reduced-fat or non-fat products leads to a reduced intake of fat-soluble bioactive substances, such as fat-soluble vitamins. Due to their natural role as transport systems for hydrophobic substances, casein micelles (CM) might depict a viable system. The structure of CM is characterized by a lipophilic core stabilized by an electric double layer-like structure. Modification allows accessibility of the core and, therefore, the inclusion of fat-soluble bioactive substances. Well-known modifications are pH reduction and use of rennet enzyme. A completely new procedure to modify CM structure is offered by pulsed electrical fields (PEF). The principle behind PEF is called electroporation and affects the electric double layer of CM so that it is interrupted. In this way, lipophilic substances can be incorporated into CM. In this work, we evaluated integration of β-carotene into native CM by an industry-compatible process to overcome disadvantages associated with the use of Na-caseinate and avoid great technical effort, e.g., due to treatment with high hydrostatic pressure. Our research has shown that PEF can be used for disintegration of CM and that significant amounts of β-carotene can be incorporated in CM. Furthermore, after disintegration using PEF, a combination of another PEF and thermal treatment was applied to restructure CM and trap significant amounts of β-carotene, permanently, ending up with an encapsulation efficiency of 78%.
Background
In mucosal barrier interfaces, flexible responses of gene expression to long-term environmental changes allow adaptation and fine-tuning for the balance of host defense and uncontrolled not-resolving inflammation. Epigenetic modifications of the chromatin confer plasticity to the genetic information and give insight into how tissues use the genetic information to adapt to environmental factors. The oral mucosa is particularly exposed to environmental stressors such as a variable microbiota. Likewise, persistent oral inflammation is the most important intrinsic risk factor for the oral inflammatory disease periodontitis and has strong potential to alter DNA-methylation patterns. The aim of the current study was to identify epigenetic changes of the oral masticatory mucosa in response to long-term inflammation that resulted in periodontitis.
Methods and results
Genome-wide CpG methylation of both inflamed and clinically uninflamed solid gingival tissue biopsies of 60 periodontitis cases was analyzed using the Infinium MethylationEPIC BeadChip. We validated and performed cell-type deconvolution for infiltrated immune cells using the EpiDish algorithm. Effect sizes of DMPs in gingival epithelial and fibroblast cells were estimated and adjusted for confounding factors using our recently developed “intercept-method”. In the current EWAS, we identified various genes that showed significantly different methylation between periodontitis-inflamed and uninflamed oral mucosa in periodontitis patients. The strongest differences were observed for genes with roles in wound healing (ROBO2, PTP4A3), cell adhesion (LPXN) and innate immune response (CCL26, DNAJC1, BPI). Enrichment analyses implied a role of epigenetic changes for vesicle trafficking gene sets.
Conclusions
Our results imply specific adaptations of the oral mucosa to a persistent inflammatory environment that involve wound repair, barrier integrity, and innate immune defense.
Background
In DNA methylation analyses like epigenome-wide association studies, effects in differentially methylated CpG sites are assessed. Two kinds of outcomes can be used for statistical analysis: Beta-values and M-values. M-values follow a normal distribution and help to detect differentially methylated CpG sites. As biological effect measures, differences of M-values are more or less meaningless. Beta-values are of more interest since they can be interpreted directly as differences in percentage of DNA methylation at a given CpG site, but they have poor statistical properties. Different frameworks are proposed for reporting estimands in DNA methylation analysis, relying on Beta-values, M-values, or both.
Results
We present and discuss four possible approaches of achieving estimands in DNA methylation analysis. In addition, we present the usage of M-values or Beta-values in the context of bioinformatical pipelines, which often demand a predefined outcome. We show the dependencies between the differences in M-values to differences in Beta-values in two data simulations: a analysis with and without confounder effect. Without present confounder effects, M-values can be used for the statistical analysis and Beta-values statistics for the reporting. If confounder effects exist, we demonstrate the deviations and correct the effects by the intercept method. Finally, we demonstrate the theoretical problem on two large human genome-wide DNA methylation datasets to verify the results.
Conclusions
The usage of M-values in the analysis of DNA methylation data will produce effect estimates, which cannot be biologically interpreted. The parallel usage of Beta-value statistics ignores possible confounder effects and can therefore not be recommended. Hence, if the differences in Beta-values are the focus of the study, the intercept method is recommendable. Hyper- or hypomethylated CpG sites must then be carefully evaluated. If an exploratory analysis of possible CpG sites is the aim of the study, M-values can be used for inference.
Background
Postoperative delirium (POD) and postoperative cognitive dysfunction (POCD) are frequent and serious complications after surgery. We aim to investigate the association between genetic variants in cholinergic candidate genes according to the Kyoto encyclopedia of genes and genomes - pathway: cholinergic neurotransmission with the development of POD or POCD in elderly patients.
Methods
This analysis is part of the European BioCog project (www.biocog.eu), a prospective multicenter observational study with elderly surgical patients. Patients with a Mini-Mental-State-Examination score ≤ 23 points were excluded. POD was assessed up to seven days after surgery using the Nursing Delirium Screening Scale, Confusion Assessment Method and a patient chart review. POCD was assessed three months after surgery with a neuropsychological test battery. Genotyping was performed on the Illumina Infinium Global Screening Array. Associations with POD and POCD were analyzed using logistic regression analysis, adjusted for age, comorbidities and duration of anesthesia (for POCD analysis additionally for education). Odds ratios (OR) refer to minor allele counts (0, 1, 2).
Results
745 patients could be included in the POD analysis, and 452 in the POCD analysis. The rate of POD within this group was 20.8% (155 patients), and the rate of POCD was 10.2% (46 patients). In a candidate gene approach three genetic variants of the cholinergic genes CHRM2 and CHRM4 were associated with POD (OR [95% confidence interval], rs8191992: 0.61[0.46; 0.80]; rs8191992: 1.60[1.22; 2.09]; rs2067482: 1.64[1.10; 2.44]). No associations were found for POCD.
Conclusions
We found an association between genetic variants of CHRM2 and CHRM4 and POD. Further studies are needed to investigate whether disturbances in acetylcholine release and synaptic plasticity are involved in the development of POD.