Open Access

The production of food-grade substances and complex biocatalysts used as additives or active ingredients – mainly for food applications – can be achieved in the eukaryotic expression system of Aspergillus niger. Food proteins or food enzymes e.g., casein, ovalbumin, phytase or glucoamylase are highly complex polymers. Most of them could be used as nitrogen or energy source for animals and humans, while others are industrial relevant biomass-degrading enzymes used for biological waste processing and food production. However, the successful production of novel recombinant proteins can be challenging, resource- and time consuming. Therefore, A. niger mutant libraries are needed to understand the “adjusting screws” to produce high yields of recombinant proteins, preferably even in a kind of generic, transferable system. In order to establish a universal and multipurpose expression platform, there is the need to overcome the lack of high throughput assays first. To tackle this problem, we designed a modular, quantitative and feasible high-throughput screening system to express and screen recombinant proteins regarding their stability and functionality in A. niger. For this purpose a dual-luciferase reporter gene system, which is applicable in small scale will be established for A. niger. After the generation of an A. niger secretion mutant library, the system will be transferred and tested to other proteins of interest. The technology can be integrated into bio-regenerative life support systems for the autonomous production of e.g., food, food additives and food enzymes on earth as well as in deep-space.

Objectives Among varied challenges of COVID-19, challenges in food and nutrition security world-over are critical. We compared the nutritional policy responses in India and Germany since both countries differ on the Human Development Index, yet both have committed to the G20 common policy response to COVID-19, besides the comparability of two large and heterogeneously populated countries, both having democratic governments. Policy research publications were reviewed using Methods qualitative meta-policy approach. We used comparative case-study. Recent food and nutrition policies of G20 nations of India and Germany were evaluated. India has primarily targeted her public distribution system and Germany has primarily targeted her food markets in order to Results manage the food and nutrition security in response to COVID-19. Both countries are coordinating additional associated nutritional policies, policies and strategies to effect an integrated sectoral approach to COVID-19 management. Both are using corrective measures of the process management strategies as well. However, the Indian management of micronutrient security for her population has over COVID-19 times acerbated and the German loan management to nutrition and agricultural small-scale industry appears to be functioning sub-optimally. Conclusions Our analysis indicates both India and Germany have responded to COVID-19 in a timely and appropriate manner regarding the food and nutrition security measures. Even so not all measures employed to tackle COVID-19 food and nutrition security have been effectively implemented, It appears, that both countries are using integrated policy in their nutrition and food security response to COVID-19.