Open Access

Raul Javier Morales Orellana, Jonas Credner, Thomas Rath

• The study of laser wounding in rose cuttings has helped to identify relevant aspects that wounded regions have to bring a positive effect on root stimulation. Nevertheless, a detailed understanding of the dynamic effects of laser wounds during rooting is not possible by traditional methods. To analyze the morphological development of rooting exposed to laser treatments, a hydroponic propagation chamber was constructed that included an internal monitoring system. Through this system, time-lapse videos recorded the entire adventitious rooting process. Results revealed how wounding influences spatial positioning of adventitious roots, depending on factors such as laser pattern, tissue removal, and the location of buds and leaves. A comparison of root distribution in terms of percentage, performed with both, the image analysis and the manual method, showed the same root growth trend with slight differences in the percentages obtained by both methods.