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Towards automated phenotyping in plant tissue culture: in situ fluorescence monitoring

  • Plant in vitro culture techniques are fundamental for research, propagation, and breeding. Automated phenotyping of in vitro cultures can revolutionize trait evaluation by transitioning to continuous and objective quantification, as well as by enhancing accuracy, speed, and efficiency. Limited research exists on automated sensor usage in plant tissue culture, mainly focusing on "plant-to-sensor" approaches. While reflection-based imaging techniques have dominated research to date, fluorescence-based imaging could offer advantages for the application of phenotyping in commercial in vitro propagation and plant research. We developed a new detector head for our “Phenomenon” plant phenotyping system to investigate the potential of fluorescence-based in situ monitoring of plant in vitro culture.

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Author:Hans BethgeORCiD, Anna Marie Tapia León, Philipp Rüter, Thomas RathORCiD, Dag Heinemann, Traud WinkelmannORCiD
Title (English):Towards automated phenotyping in plant tissue culture: in situ fluorescence monitoring
DOI:https://doi.org/10.1117/12.2692924
Parent Title (English):Proceedings
Document Type:Conference Proceeding
Language:English
Year of Completion:2024
Creating Corporation:SPIE
Release Date:2024/06/24
Tag:Fluorecence Monitoring; Phenotyping
Volume:12879
First Page:72
Last Page:78
Note:
SPIE LASE | 27.01. - 01.02. 2024, Photonic Technologies in Plant and Agricultural Science, San Francisco, California, United States
Faculties:Fakultät AuL
DDC classes:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften, Biologie
Collections:nur Publikationsnachweis
Review Status:Peer Reviewed