Volltext-Downloads (blau) und Frontdoor-Views (grau)

Epigenetic adaptations of the masticatory mucosa to periodontal inflammation

  • Background In mucosal barrier interfaces, flexible responses of gene expression to long-term environmental changes allow adaptation and fine-tuning for the balance of host defense and uncontrolled not-resolving inflammation. Epigenetic modifications of the chromatin confer plasticity to the genetic information and give insight into how tissues use the genetic information to adapt to environmental factors. The oral mucosa is particularly exposed to environmental stressors such as a variable microbiota. Likewise, persistent oral inflammation is the most important intrinsic risk factor for the oral inflammatory disease periodontitis and has strong potential to alter DNA-methylation patterns. The aim of the current study was to identify epigenetic changes of the oral masticatory mucosa in response to long-term inflammation that resulted in periodontitis. Methods and results Genome-wide CpG methylation of both inflamed and clinically uninflamed solid gingival tissue biopsies of 60 periodontitis cases was analyzed using the Infinium MethylationEPIC BeadChip. We validated and performed cell-type deconvolution for infiltrated immune cells using the EpiDish algorithm. Effect sizes of DMPs in gingival epithelial and fibroblast cells were estimated and adjusted for confounding factors using our recently developed “intercept-method”. In the current EWAS, we identified various genes that showed significantly different methylation between periodontitis-inflamed and uninflamed oral mucosa in periodontitis patients. The strongest differences were observed for genes with roles in wound healing (ROBO2, PTP4A3), cell adhesion (LPXN) and innate immune response (CCL26, DNAJC1, BPI). Enrichment analyses implied a role of epigenetic changes for vesicle trafficking gene sets. Conclusions Our results imply specific adaptations of the oral mucosa to a persistent inflammatory environment that involve wound repair, barrier integrity, and innate immune defense.

Download full text files

Export metadata

Additional Services

Search Google Scholar

Statistics

frontdoor_oas
Metadaten
Author:Gesa Marijke Richter, Jochen Kruppa-ScheetzORCiD, Huseyin Gencay KeceliORCiD, Emel Tuğba Ataman-DuruelORCiD, Christian GraetzORCiD, Nicole PischonORCiD, Gunar WagnerORCiD, Carsten RendenbachORCiD, Yvonne Jockel-SchneiderORCiD, Orlando MartinsORCiD, Corinna BruckmannORCiD, Ingmar StaufenbielORCiD, Andre Franke, Rahime M. NohutcuORCiD, Søren JepsenORCiD, Henrik DommischORCiD, Arne S. SchaeferORCiD
Title (English):Epigenetic adaptations of the masticatory mucosa to periodontal inflammation
URN:urn:nbn:de:bsz:959-opus-58932
DOI:https://doi.org/10.1186/s13148-021-01190-7
ISSN:1868-7083
ISSN:1868-7075
Parent Title (English):Clinical Epigenetics
Document Type:Article
Language:English
Year of Completion:2021
Release Date:2024/04/23
Tag:Cell type deconvolution; EWAS; Gingiva; Inflammation; Methylation; PTP4A3; Periodontitis; ROBO2
Issue:13
Article Number:203
Page Number:22
Faculties:Fakultät AuL
DDC classes:600 Technik, Medizin, angewandte Wissenschaften / 610 Medizin, Gesundheit
Review Status:Veröffentlichte Fassung/Verlagsversion
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International