TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Strohbach, Anne
A1  - Maess, Friedemann
A1  - Wulf, Katharina
A1  - Petersen, Svea
A1  - Grabow, Niels
A1  - Schmitz, Klaus-Peter
A1  - Felix, Stephan B.
A1  - Busch, Raila
T1  - The Role of Biodegradable Poly-(L-lactide)-Based Polymers in Blood Cell Activation and Platelet-Monocyte Interaction
JF  - International Journal of Molecular Sciences
N2  - The main purpose of new stent technologies is to overcome unfavorable material-related incompatibilities by producing bio- and hemo-compatible polymers with anti-inflammatory and anti-thrombogenic properties. In this context, wettability is an important surface property, which has a major impact on the biological response of blood cells. However, the influence of local hemodynamic changes also influences blood cell activation. Therefore, we investigated biodegradable polymers with different wettability to identify possible aspects for a better prediction of blood compatibility. We applied shear rates of 100 s−1 and 1500 s−1 and assessed platelet and monocyte activation as well as the formation of CD62P+ monocyte-bound platelets via flow cytometry. Aggregation of circulating platelets induced by collagen was assessed by light transmission aggregometry. Via live cell imaging, leukocytes were tracked on biomaterial surfaces to assess their average velocity. Monocyte adhesion on biomaterials was determined by fluorescence microscopy. In response to low shear rates of 100 s−1, activation of circulating platelets and monocytes as well as the formation of CD62P+ monocyte-bound platelets corresponded to the wettability of the underlying material with the most favorable conditions on more hydrophilic surfaces. Under high shear rates, however, blood compatibility cannot only be predicted by the concept of wettability. We assume that the mechanisms of blood cell-polymer interactions do not allow for a rule-of-thumb prediction of the blood compatibility of a material, which makes extensive in vitro testing mandatory.
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:959-opus-68148
SN  - 1422-0067
SS  - 1422-0067
U6  - https://doi.org/10.3390/ijms22126340
DO  - https://doi.org/10.3390/ijms22126340
N1  - Open Access
VL  - 22
IS  - 12
PB  - MDPI AG
ER  -