@article{PetersenDemannRestemeyeretal.2021, author = {Petersen, Finn and Demann, Johannes and Restemeyer, Dina and Ulbrich, Andreas and Olfs, Hans-Werner and Westendarp, Heiner and Appenroth, Klaus-J{\"u}rgen}, title = {Influence of the Nitrate-N to Ammonium-N Ratio on Relative Growth Rate and Crude Protein Content in the Duckweeds Lemna minor and Wolffiella hyalina}, journal = {Plants}, volume = {10}, number = {8}, issn = {2223-7747}, doi = {10.3390/plants10081741}, institution = {Fakult{\"a}t AuL}, pages = {1741}, year = {2021}, abstract = {In order to produce protein-rich duckweed for human and animal consumption, a stable cultivation process, including an optimal nutrient supply for each species, must be implemented. Modified nutrient media, based on the N-medium for duckweed cultivation, were tested on the relative growth rate (RGR) and crude protein content (CPC) of Lemna minor and Wolffiella hyalina, as well as the decrease of nitrate-N and ammonium-N in the media. Five different nitrate-N to ammonium-N molar ratios were diluted to 10\% and 50\% of the original N-medium concentration. The media mainly consisted of agricultural fertilizers. A ratio of 75\% nitrate-N and 25\% ammonium-N, with a dilution of 50\%, yielded the best results for both species. Based on the dry weight (DW), L. minor achieved a RGR of 0.23 ± 0.009 d-1 and a CPC of 37.8 ± 0.42\%, while W. hyalina's maximum RGR was 0.22 ± 0.017 d-1, with a CPC of 43.9 ± 0.34\%. The relative protein yield per week and m2 was highest at this ratio and dilution, as well as the ammonium-N decrease in the corresponding medium. These results could be implemented in duckweed research and applications if a high protein content or protein yield is the aim.}, language = {en} }